Blastocystis sp.

Chai, Yijun, Deng, Lei, Liu, Haifeng, Yao, Jingxin, Zhong, Zhijun, Fu, Hualin, Shen, Liuhong, Zhou, Ziyao, Deng, Junliang, Hu, Yanchun & Peng, Guangneng, 2020, First subtyping of Blastocystis sp. from pet rodents in southwestern China, International Journal for Parasitology: Parasites and Wildlife 11, pp. 143-148 : 145

2.5. Subtyping of Blastocystis sp.

All DNA preparations were screened for the presence of Blastocystis sp. by PCR amplification of the barcode region (a fragment of ~510 bp) of the SSU rRNA gene. The primers and cycling parameters were in accordance to those described by Scicluna et al. (2006). TaKaRa Taq DNA polymerase (TaKaRa Bio Inc., Tokyo, Japan) was used for all of the PCR reactions. A negative control with no DNA added was included in all of the PCR tests. PCR products were subjected to electrophoresis in a 1.5% agarose gel and visualized by staining the gel with ethidium bromide .