Blastocystis sp.

Chai, Yijun, Deng, Lei, Liu, Haifeng, Yao, Jingxin, Zhong, Zhijun, Fu, Hualin, Shen, Liuhong, Zhou, Ziyao, Deng, Junliang, Hu, Yanchun & Peng, Guangneng, 2020, First subtyping of Blastocystis sp. from pet rodents in southwestern China, International Journal for Parasitology: Parasites and Wildlife 11, pp. 143-148 : 146

3.2. Distribution of Blastocystis sp. subtypes in pet rodents

Two Blastocystis sp. subtypes were successfully sequenced, and all SSU rRNA –positive PCR samples were sequenced and phylogenetically analyzed ( Fig. 2 ). Two subtypes (ST4 and ST17) were identified based on the phylogenetic tree (ST4, ST17), both of which are known to have zoonotic potential ( Table 1, Fig. 2 ). ST4 was the predominant subtype, and was widely distributed among the different species of rodents and locations ( Table 1). Additionally, ST17 was discovered in a Chinchilla , which is a newly identified host for this subtype.

Figures

Fig. 2. Phylogenetic relationships among nucleotide sequences of Blastocystis partial small subunit ribosomal RNA (SSU rRNA) genes. The neighbor-joining method was used to construct the trees from the Kimura-2- parameter model. Branch numbers represent percent bootstrapping values from 1000 replicates, with values of more than 50% shown in the tree. Each sequence is identified by its accession number, subtypes, host origin, and country. Blastocystis subtypes identified in the present study are indicated in bold-type.▲ are subtypes in this study.