Heterodera latipons

İmren, Mustafa, B, Şenol Yildiz, B, Halil Toktay, Duman, Nagihan & Dababat B, Abdelfattah A., 2018, Morphometric and genetic variability among Mediterranean cereal cyst nematode (Heterodera latipons) populations in Turkey, Turkish Journal of Zoology 42 (6), pp. 625-636 : 626

2.1. Heterodera latipons populations

A total of 42 Heterodera populations were collected from wheat and barley fields in Turkey during the 2016 growing season. Of these, 26 populations were collected from the southeastern area (Gaziantep, Kilis, and Mardin provinces) and 16 populations from the eastern Mediterranean region (Adana and Hatay provinces), as shown in Table 1 .

The wheat and barley fields surveyed displayed stunted patches, poor plant growth, chlorotic lower leaves, and few or no tillers. Cyst populations were collected from 42 different fields arbitrarily with separation distance of 7–10 km. A representative sample of 2 kg of soil consisting of 5–7 subsamples was prepared. A modified sieving-decanting method ( Fenwick, 1940) was used to extract cysts from the soil. Cysts were identified to genus level under a stereobinocular microscope (V20, Zeiss, Jena, Germany). At least ten cysts were collected for each population and stored at 4 °C for use in molecular and morphological analysis.