Aedes aegypti

Shah, Farooq A., Abdoarrahem B, Mostafa M., B, Colin Berry, B, Mustapha Touray, B, Selcuk Hazir & Butt B, Tariq M., 2021, Indiscriminate ingestion of entomopathogenic nematodes and their symbiotic bacteria by Aedes aegypti larvae: a novel strategy to control the vector of Chikungunya, dengue and yellow fever, Turkish Journal of Zoology 45, pp. 372-383 : 373

2.1. Maintenance of Aedes aegypti

Wild-type adult Ae. aegypti were reared at Cardiff School of Biosciences , Cardiff University , Cardiff, UK. Adult mosquitoes were maintained in cages kept in a controlled temperature room at 30 °C with a 12 h light: 12 h dark photoperiod. Mosquitoes were fed every 2 days on defibrinated horse blood ( TSC Biosciences Ltd, Buckingham, UK) using an artificial blood feeder (Hemotek membrane feeding systems, Lancs. England). Mosquitoes laid eggs on Whatman 3 MM papers in waterfilled cups. Eggs were stored at 4 °C until required or hatched in tap water in small plastic containers (10 × 15 × 15 cm). Eggs hatched within 24 h. Larvae were fed on crushed rabbit pellets and maintained at 22 ± 2 ° C. Each larval stage lasted for 2–3 days, thereby, pupal stages were observed 10–14 days after the emergence of the larvae .