Ctenocolum aquilus Albuquerque & Ribeiro-Costa

Albuquerque, Felícia Pereira De, Manfio, Daiara & Ribeiro-Costa, Cibele Stramare, 2014, A contribution to the knowledge of New World Bruchinae (Coleoptera, Chrysomelidae): taxonomic revision of Ctenocolum Kingsolver & Whitehead, with description of five new species, Zootaxa 3838 (1), pp. 1-45 : 21-24

Ctenocolum aquilus Albuquerque & Ribeiro-Costa sp. nov.

( Figs. 8 , 22 , 35 , 53 , 66 , 79 , 91 )

Type material. Holotype deposited in DZUP, male: “GUARAPUAVA – PARANÁ/ Est.Águas Sta.Clara/ BRASIL 0 2. I. 1987 / Lev. Ent. PROFAUPAR/ LÂMPADA” [white label printed in black]; “♂” [white label printed in black]; “ HOLOTYPE / Ctenocolum aquilus / Det. Albuquerque & Ribeiro-Costa” [white label with red margin, first line printed in red, remainder printed in black]. 32 Paratypes deposited in the following collections: 12 in DZUP, 1 male: “Porto Ferreira, SP/ Reserva Estadual – IF/ 28.IX.94 / E.P. Teixeira–col." [white label with margin and E.P. Teixeira–col. printed in black, remainder handwritten in black]; "Hosp. Lonchocarpus / muehlbergianus / Hassl./ (loncocarpus)” [white label with margin printed in black, handwritten in black]; “♂” [white label printed in black]; “ PARATYPE / Ctenocolum aquilus / Det. Albuquerque & Ribeiro-Costa” [white label with yellow margin, printed in black]. 2 males: “JUNDIAÍ DO SUL – PR/ Fazenda Monte Verde/ BRASIL 29.XII.1986 / Lev. Ent.

PROFAUPAR/ LÂMPADA” [white label printed in black]; “♂” [white label printed in black]; “ PARATYPE / Ctenocolum aquilus / Det. Albuquerque & Ribeiro-Costa” [white label with yellow margin, printed in black]. 1 female: “JUNDIAÍ DO SUL – PR/ Fazenda Monte Verde/ BRASIL 29.XII.1986 / Lev. Ent. PROFAUPAR/ LÂMPADA” [white label printed in black]; “♀” [white label printed in black]; “ PARATYPE / Ctenocolum aquilus / Det. Albuquerque & Ribeiro-Costa” [white label with yellow margin, printed in black]. 1 female: “JUNDIAÍ DO SUL – PR/ Fazenda Monte Verde/ BRASIL 31.XII.1986 / Lev. Ent. PROFAUPAR/ LÂMPADA” [white label printed in black]; “♀” [white label printed in black]; “ PARATYPE / Ctenocolum aquilus / Det. Albuquerque & Ribeiro-Costa” [white label with yellow margin, printed in black]. 1 female: “TELÊMACO BORBA – PR/ Res. Samuel Klabin/ BRASIL 31.III.1987 / Lev. Ent. PROFAUPAR/ LÂMPADA” [white label printed in black]; “♀” [white label printed in black]; “ PARATYPE / Ctenocolum aquilus / Det. Albuquerque & Ribeiro-Costa” [white label with yellow margin, printed in black]. 1 male: “PR. Três Barras/ 22.VII.1999 / Maschio, W. col.” [white label with margin printed in black, handwritten in black]; “♂” [white label printed in black]; “ PARATYPE / Ctenocolum aquilus / Det. Albuquerque & Ribeiro-Costa” [white label with yellow margin, printed in black]. 1 female: "PR.Três Barras/ 22.VII.1999 / Maschio, W. col." [white label with margin printed in black, handwritten in black]; “♀” [white label printed in black]; “ PARATYPE / Ctenocolum aquilus / Det. Albuquerque & Ribeiro- Costa” [white label with yellow margin, printed in black]. 1 male: “ BRASIL.PR.Três Barras/ 22.julho.1999/ Maschio, W. col.” [white label with margin printed in black, handwritten in black]; “ Ctenocolum / sp./ Ribeiro-Costa, 2000” [white label with margin printed in black, handwritten in black]; “♂” [white label printed in black]; “ PARATYPE / Ctenocolum aquilus / Det. Albuquerque & Ribeiro-Costa” [white label with margin yellow and printed in black]. 1 male: “PR.Três Barras/ 22.VII 1999 / Maschio,W. col.” [white label with margin printed in black, handwritten in black]; “ Ctenocolum / crotonae / ( Fahraeus, 1839)” [white label with margin printed in black, handwritten in black]; “Pl. Hosp./ Lonchocarpus / muehlbergianus ” [white label with margin printed in black, handwritten in black]; “♂” [white label printed in black]; “ PARATYPE / Ctenocolum aquilus / Det. Albuquerque & Ribeiro-Costa” [white label with yellow margin, printed in black]. 1 male: “GUARAPUAVA – PARANÁ/ Est. Águas Sta. Clara/ BRASIL 29.XI.1986 / Lev. Ent. PROFAUPAR/ LÂMPADA” [white label printed in black]; “♂” [white label printed in black]; “ PARATYPE / Ctenocolum aquilus / Det. Albuquerque & Ribeiro-Costa” [white label with yellow margin, printed in black]. 1 female: “GUARAPUAVA – PARANÁ/ Est.Águas Sta.Clara/ BRASIL 27.I.1987 / Lev. Ent. PROFAUPAR/ LÂMPADA” [white label printed in black]; “♀” [white label printed in black]; “ PARATYPE / Ctenocolum aquilus / Det. Albuquerque & Ribeiro-Costa” [white label with yellow margin, printed in black]. 3 in FSCA, 1 male: “ BRASIL.PR.Três Barras/ 17.julho.1999/ Maschio.W. col.” [white label with margin printed in black, handwritten in black]; “♂” [white label printed in black]; “ PARATYPE / Ctenocolum aquilus / Det. Albuquerque & Ribeiro-Costa” [white label with yellow margin, printed in black]. “ 1 female: BRASIL.PR.Três Barras/ 17.julho.1999/ Maschio.W. col.” [white label with margin printed in black, handwritten in black]; “♀” [white label printed in black]; “ PARATYPE / Ctenocolum aquilus / Det. Albuquerque & Ribeiro-Costa” [white label with yellow margin, printed in black]. 1 female: "PR.Três Barras/ 22.VII.1999 / Maschio, W. col." [white label with margin printed in black, handwritten in black]; “♀” [white label printed in black]; “ PARATYPE / Ctenocolum aquilus / Det. Albuquerque & Ribeiro-Costa” [white label with yellow margin, printed in black]. 3 in MNRJ, 1 male: “PR.Três Barras/ 22.VII.1999 / Mashio, W.col.” [white label with margin printed in black, handwritten in black]; “♂” [white label printed in black]; “ PARATYPE / Ctenocolum aquilus / Det. Albuquerque & Ribeiro- Costa” [white label with yellow margin, printed in black]. 1 female: “PR.Três Barras/ 22.VII.1999 / Mashio, W.col.” [white label with margin printed in black, handwritten in black]; “♀” [white label printed in black]; “ PARATYPE / Ctenocolum aquilus / Det. Albuquerque & Ribeiro-Costa” [white label with yellow margin, printed in black]. 1 female: “ BRASIL.PR.Três Barras/17.julho.1999/ Maschio, W. col.” [white label with margin printed in black, handwritten in black]; “♀” [white label printed in black]; “ PARATYPE / Ctenocolum aquilus / Det. Albuquerque & Ribeiro-Costa” [white label with yellow margin, printed in black]. 3 in MZSP, 1 female: “JUNDIAÍ DO SUL – PR/ Fazenda Monte Verde/ BRASIL 29.XII.1986 / Lev. Ent. PROFAUPAR/ LÂMPADA” [white label printed in black]; “♀” [white label printed in black]; “ PARATYPE / Ctenocolum aquilus / Det. Albuquerque & Ribeiro-Costa” [white label with yellow margin, printed in black]. 1 male: “PR. Três Barras/ 22.VII.1999 / Mashio, W.col.” [white label with margin printed in black, handwritten in black]; “♂” [white label printed in black]; “ PARATYPE / Ctenocolum aquilus / Det. Albuquerque & Ribeiro-Costa” [white label with yellow margin, printed in black]. 1 female: “PR. Três Barras/ 22.VII.1999 / Mashio, W.col.” [white label with margin printed in black, handwritten in black]; “♀” [white label printed in black]; “ PARATYPE / Ctenocolum aquilus / Det. Albuquerque & Ribeiro-Costa” [white label with yellow margin, printed in black]. 4 in TAMU, 1 male: “ BRASIL.PR.Três Barras/17.julho.1999/ Maschio.W. col.” [white label with margin printed in black, handwritten in black]; “♂” [white label printed in black]; “ PARATYPE / Ctenocolum aquilus / Det. Albuquerque & Ribeiro-Costa” [white label with yellow margin, printed in black]. 1 female: “ BRASIL.PR.Três Barras/ 17.julho.1999/ Maschio.W. col.” [white label with margin printed in black, handwritten in black]; “♀” [white label printed in black]; “ PARATYPE / Ctenocolum aquilus / Det. Albuquerque & Ribeiro-Costa” [white label with yellow margin, printed in black]. 1 male: “GUARAPUAVA – PARANÁ/ Est. Águas Sta.Clara/ BRASIL 01.XI.1986 / Lev. Ent. PROFAUPAR/ LÂMPADA” [white label printed in black]; “ Ctenocolum / crotonae / ( Fhraeus, 1839)” [white label with margin printed in black, handwritten in black]; “♂” [white label printed in black]; “ PARATYPE / Ctenocolum aquilus / Det. Albuquerque & Ribeiro-Costa” [white label with yellow margin, printed in black]. 1 female: GUARAPUAVA – PARANÁ/ Est.Águas Sta.Clara/ BRASIL 0 2. I. 1987 / Lev. Ent. PROFAUPAR/ LÂMPADA” [white label printed in black]; “♀” [white label printed in black]; “ PARATYPE / Ctenocolum aquilus / Det. Albuquerque & Ribeiro - Costa." [white label with yellow margin printed in black]. 4 in USNM, 2 females: "PR.Três Barras/ 22.VII.1999 / Maschio, W. col." [white label with margin printed in black, handwritten in black]; “♀” [white label printed in black]; “ PARATYPE / Ctenocolum aquilus / Det. Albuquerque & Ribeiro-Costa” [white label with yellow margin, printed in black]. 2 males: “GUARAPUAVA – PARANÁ/ Est.Águas Sta.Clara/ BRASIL 0 2. I. 1987 / Lev. Ent. PROFAUPAR/ LÂMPADA” [white label printed in black]; “♂” [white label printed in black]; “ PARATYPE / Ctenocolum aquilus / Det. Albuquerque & Ribeiro-Costa” [white label with yellow margin, printed in black]. 3 in ZMHB, 2 males: “JUNDIAÍ DO SUL – PR/ Fazenda Monte Verde/ BRASIL 29.XII.1986 / Lev. Ent. PROFAUPAR/ LÂMPADA” [white label printed in black]; “♂” [white label printed in black]; “ PARATYPE / Ctenocolum aquilus / Det. Albuquerque & Ribeiro-Costa” [white label with yellow margin, printed in black]. 1 female: "PR.Três Barras/ 22.VII.1999 / Maschio, W. col." [white label with printed margin in black, handwritten in black]; “♀” [white label printed in black]; “ PARATYPE / Ctenocolum aquilus / Det. Albuquerque & Ribeiro-Costa” [white label with yellow margin, printed in black].

Diagnosis. Ctenocolum aquilus Albuquerque & Ribeiro-Costa sp. nov. differs from all other species by having antennomeres 3–11 darker at apex ( Fig. 53 ) and male genitalia at submedian region with squamous inverted Yshape sclerite ( Fig. 79 ).

Description. BL: 2.5–2.9 mm; BW: 1.7–2.0 mm.

Integument. Dorsum in general dark brown or dark brown and brown. Antenna pale brown or brown, antennomeres 3–11 darker at apex of each antennomere ( Figs. 8 , 53 ). Pygidium rufous and dark brown or pale brown. Ventral region in general black and reddish brown sometimes black, reddish brown and pale brown. Front and middle femur and tibia pale brown to brown; hind femur brown and dark brown.

Pubescence. Pronotum brown, white and black; sparse setae exposing integument forming an oval, wide area from anterior to posterior region usually divided by transversal and longitudinal strip of denser setae and on each lateral region one small oval area ( Fig. 8 ). Elytra strongly variegated with brown, white and black setae; interstria 3 with short dense strip of white setae at base and at submedian region ( Fig. 8 ). Pygidium white, dense except on two basal, four lateral and one apical small areas with sparse setae; at median region a larger area with sparse setae ( Fig. 35 ). Ventral region pale brown, brown and white ( Fig. 53 ).

Head. Ocular sinus 0.2 mm; ocular index 5.1–6.8; length of eyes in frontal view behind sinus 0.07–0.09 mm ( Fig. 22 ). Antenna serrate from antennomere 4-10 in male and from 5-10 in female. Frons with frontal carina ( Fig. 22 ).

Prothorax. Pronotum with median gibbosity slightly elevated, usually not divided by longitudinal sulcus and divided by transversal sulcus; lateral gibbosity slightly to moderately elevated; basal lobe with depression and slightly emarginated ( Fig. 8 ).

Mesothorax and metathorax. Elytra, striae with punctures moderately impressed; less conspicuous teeth at base of striae 3 and 4; tooth of stria 4 closer to base of tooth of stria 3 than to anterior margin of elytra; stria 6 conspicuously impressed. Hind femur ( Fig. 66 ) on external ventral margin with toothed carina; without denticles above external ventral margin; pecten with 6–8 teeth. Hind tibia ( Fig. 66 ) strongly emarginated beside mucro; lateral coronal denticles present.

Abdomen. Pygidium longer than wide, oval, at median basal region with moderately impressed punctures ( Fig. 35 ).

Male genitalia. Median lobe with ventral valve as long as wide, lateral margin concave on subapical region ( Fig. 79 ), basal margin slightly emarginated. Internal sac, lateral apex with short tuft of setae, hinge sclerite curved at base, short, extending over 1/3 of subapical region; subapical region with homogeneously distributed spicules, dense on lateral elongated strip; median region with homogeneously distributed spicules; submedian region with squamous inverted Y-shape sclerite ( Fig. 79 ); basal region without denticles or spines. Tegmen ( Fig. 91 ), lateral lobes separated by emargination about 0.7 times the length of lateral lobes; internal margin near end of emargination straight, forming a “V”; expanded at apex, about 2 times the smallest width on median region; without membranous projection at apex.

Etymology. The specific name " aquilus " refers to the antennomeres 3–11 with darker apex.

Distribution. Neotropical region: Brazil (São Paulo, Paraná).

Host plant (Tables I–II). Papilionoideae : Lonchocarpus muehlbergianus Hassl.

Note. The species mentioned as C. podagricus by Sari et al (2002) belongs to C. aquilus Albuquerque & Ribeiro-Costa sp. nov. In their paper, the authors Sari et al. (2002), performed a survey of insects associated with seeds of Lonchocarpus muehlbergianus , recorded from a total of 2,353 examined seeds, 4.9% damaged by C. aquilus Albuquerque & Ribeiro-Costa sp. nov. (= C. podagricus ) and 4.6% by Horismenus missouriensis . According to Sari et al. (2002), the parasitoid H. missouriensis is probably associated with C. aquilus Albuquerque & Ribeiro-Costa sp. nov. (= C. podagricus ).

Figures

FIGURES 8 – 16. Dorsal habitus: 8 Ctenocolum aquilus Albuquerque & Ribeiro-Costa sp. nov. (paratype); 9 C. biolleyi (holotype); 10 C. colburni; 11 C. martiale (paratype), 12, C. martiale; 13 C. milelo Albuquerque & Ribeiro-Costa sp. nov. (paratype); 14, C. podagricus; 15 C. punctinotatus Albuquerque & Ribeiro-Costa sp. nov. (holotype); 16 C. pygospilotos Albuquerque & Ribeiro-Costa sp. nov. (holotype). Scale: 0.50 mm.

FIGURES 22 – 30. Frontal habitus: 22 Ctenocolum aquilus Albuquerque & Ribeiro-Costa sp. nov. (paratype); 23 C. biolleyi (paratype); 24 C. colburni; 25 C. martiale; 26 C. milelo Albuquerque & Ribeiro-Costa sp. nov. (paratype); 27 C. podagricus; 28 C. punctinotatus Albuquerque & Ribeiro-Costa sp. nov. (holotype); 29 C. pygospilotos Albuquerque & Ribeiro-Costa sp. nov. (holotype); 30 C. triangulatus Albuquerque & Ribeiro-Costa sp. nov. (holotype). Scale: 0.50 mm.

FIGURES 35 – 43. Pygidium of male: 35 Ctenocolum aquilus Albuquerque & Ribeiro-Costa sp. nov. (paratype); 36 C. colburni; 37 C. martiale; 38 C. milelo Albuquerque & Ribeiro-Costa sp. nov. (paratype); 39 C. podagricus; 40 C. punctinotatus Albuquerque & Ribeiro-Costa sp. nov. (holotype); 41 C. pygospilotos Albuquerque & Ribeiro-Costa sp. nov. (holotype); 42 C. triangulatus Albuquerque & Ribeiro-Costa sp. nov. (holotype); 43 C. acapulcensis. Scale: 0.50 mm.

FIGURES 53 – 60. Lateral habitus: 53 Ctenocolum aquilus Albuquerque & Ribeiro-Costa sp. nov. (paratype); 54 C. biolleyi (holotype); 55 C. colburni; 56 C. martiale (paratype); 57 C. milelo (paratype); 58 C. podagricus; 59 C. punctinotatus Albuquerque & Ribeiro-Costa sp. nov. (holotype); 60 C. pygospilotos Albuquerque & Ribeiro-Costa sp. nov. (holotype). Scale: 0.50 mm.

FIGURES 66 – 71. Posterior leg, external view: 66 Ctenocolum aquilus (paratype); 67 C. biolleyi (holotype); 68 C. colburni; 69 C. martiale; 70 C. milelo Albuquerque & Ribeiro-Costa sp. nov. (paratype); 71 C. podagricus. Scale: 0.50 mm.

FIGURES 79 – 84. Male genitalia, median lobe: 79 Ctenocolum aquilus Albuquerque & Ribeiro-Costa sp. nov. (paratype); 80 C. colburni; 81 C. martiale (holotype); 82 C. milelo (paratype); 83 C. podagricus; 84 C. punctinotatus Albuquerque & Ribeiro-Costa sp. nov. (holotype). Scale: 0.10 mm.

FIGURES 91 – 99. Male genitalia, tegmen, lateral lobe: 91 C. aquilus (paratype); 92 C. colburni; 93 C. martiale (holotype); 94 C. milelo Albuquerque & Ribeiro-Costa sp. nov. (paratype); 95 C. podagricus; 96 C. punctinotatus Albuquerque & Ribeiro-Costa sp. nov. (holotype); 97 C. pygospilotos Albuquerque & Ribeiro-Costa sp. nov. (holotype); 98 C. triangulatus Albuquerque & Ribeiro-Costa sp. nov. (holotype); 99 C. acapulcensis. Scale: 0.10 mm.