Prosimulium rachiliense, DJAFAROV

Adler, Peter H., S, Ümit & Irin, 2014, Cytotaxonomy of the Prosimulium (Diptera: Simuliidae) of Western Asia, Zoological Journal of the Linnean Society 171 (4), pp. 753-768 : 758

PROSIMULIUM RACHILIENSE DJAFAROV CYTOFORM ‘A’

Cytoform ‘A’, consisting of 83 larvae from the Caucasus Mountains of Armenia, was fixed for inversions IS- 20 and IS-21 ( Fig. 4 ) and had a sex-differential segment in the expanded centromere region of chromosome I. Males were characterized by a lack of pairing in section 19′ of the CIt region; lack of pairing ranged from imperceptible (X 0 Y 0), approaching the paired condition in females (e.g. Site 5), to complete repulsion (X 0 Y 1). Autosomal inversion IL-10 ( Fig. 6B ) was found in 15– 23% of homologues per site, and was in Hardy−Weinberg equilibrium at Site 3 ( Table 3). Four additional autosomal inversions were rare, including the perinucleolar IL-11 inversion ( Table 3; Fig. 7 ). One female had a large block of heterochromatin, 88hc(r), in one homologue ( Fig. 11 ). Ectopic pairing of centromere bands occurred frequently .

Figures

Figure 4. Chromosome arm IS of Prosimulium rachiliense cytoform ‘B’ (male). Relative to the standard sequence, IS-20 is present. Limits of inversion IS-21 of cytoform ‘A’ are indicated by a bracket. Fixed rearrangements are italicized and polymorphic rearrangements are in standard type. Abbreviations: C, centromere; “3”, 3 heavy marker.

Figure 6. Chromosome arm IL. A, Prosimulium frontatum (female; sections 23–44). Relative to the standard sequence, IL-12 is present. B, base of chromosome IL of Prosimulium rachiliense cytoform ‘A’ (male) with the IL-10 sequence. Fixed rearrangements are italicized and polymorphic rearrangements are in standard type. Abbreviation: N.O, nucleolar organizer.

Figure 7. Chromosome arm IL of Prosimulium tomosvaryi (female), showing the standard sequence. Limits of IL-11 of Prosimulium rachiliense ‘A’ and IL-13 and IL-14 of P. rachiliense ‘B’ are shown by brackets. Abbreviations: hb, location of heteroband 23hb of P. tomosvaryi; N.O., nucleolar organizer.

Figure 11. Chromosome arm IIIL of Prosimulium rachiliense cytoform ‘A’ (male), showing the standard sequence. Limits of IIIL-21–IIIL-24 of P. rachiliense ‘A’, IIIL-25 of Prosimulium frontatum, and IIIL-26 and IIIL-27 of Prosimulium tomosvaryi are indicated by brackets. Fixed rearrangements are italicized and polymorphic rearrangements are in standard type. Abbreviations: C, centromere; hb, location of heteroband 84hb of P. tomosvaryi; hc, location of extra heterochromatin (85hc) of P. tomosvaryi; hc(r) and hc(t), extra heterochromatin of P. rachiliense ‘A’ and P. tomosvaryi, respectively; s, shield; t, triad.