Hymenobacter aquatilis, Kang & Cha & Kim & Joh, 2018

Kang, Heeyoung, Cha, Inseong, Kim, Haneul & Joh, Kiseong, 2018, Hymenobacter aquatilis sp. nov., isolated from a mesotrophic artificial lake, International Journal of Systematic and Evolutionary Microbiology 68 (6), pp. 2036-2041 : 2040

DESCRIPTION OF HYMENOBACTER AQUATILIS SP. NOV.

Hymenobacter aquatilis (a.qua′ ti.lis. L. masc. adj. aquatilis aquatic, living in water).

Cells are strictly aerobic, Gram-stain-negative, non-motile rods, approximately 0.5–0.8 µm wide and 1.7–3.0 µm long. Colonies on R2A agar are irregular, wavy, umbonate, pale red-coloured and approximately 4–6 mm in diameter after 2 days at 30 Ǫ C. Growth occurs at 10–37 Ǫ C (optimum, 25– 30 Ǫ C), at pH 7–8 (optimum, pH 7.0) and in R2A broth supplemented with 0–0.5 % NaCl (optimum, 0 % NaCl). Good growth occurs on NA, but not on blood, marine, TSA or MacConkey agars. Casein (skimmed milk) and starch are hydrolysed. DNA, dextrin, CM-cellulose and cellulose are not hydrolysed. Susceptible to ampicillin, chloramphenicol, erythromycin, rifampicin, tetracycline and vancomycin, but resistant to gentamicin, kanamycin, penicillin G and streptomycin. In the API 20NE strip, positive results are obtained for aesculin and gelatin hydrolyses, and β -galactosidase (PNPG test) activity and assimilation of D- glucose, D- mannose and maltose, but negative results for nitrate reduction, indole production, glucose ferementation, arginine dihydrolase and urease activities, and assimilation of L- arabinose, D- mannitol, N -acetyl-glucosamine, potassium gluconate, capric acid, adipic acid, malic acid, trisodium citrate and phenylacetic acid. In the API ZYM strip, positive results are obtained for alkaline phosphatase, esterase (C4), esterase lipase (C8), leucine arylamidase, valine arylamidase, cystine arylamidase, trypsin, acid phosphatase, naphthol-AS- BI-phosphohydrolase and Oi -glucosidase and N - acetylβ -glucosaminidase activities are present; lipase (C14), Oi -chymotrypsin, Oi -galactosidase, β -galactosidase, β -glucuronidase, β -glucosidase, Oi -mannosidase and Oi -fucosidase activities are absent. In the GN2 Biolog MicroPlate, Oi -cyclodextrin, glycogen, adonitol, cellobiose, L- fucose, Oi -D- glucose, maltose, D- mannose, methyl β -D- glucoside, D- psicose, D- sorbitol, sucrose, trehalose, turanose, pyruvic acid methyl ester, succinic acid mono-methyl-ester, acetic acid, Oi -ketobutyric acid, propionic acid, succinic acid, bromosuccinic acid, L- alanine, L- glutamic acid, glycyl-L- aspartic acid, glycyl-L- glutamic acid, L- ornithine, L- proline, L- threonine, D, L- carnitine, Ƴ -amino butyric acid, Oi -D- glucose-1-phosphate and D- glucose-6-phosphate are utilized. Other substrates are not utilized (Table S1). The major cellular fatty acids are iso-C 15: 0, C 16: 1 Ɯ 5 c, summed feature 4 (iso-C 17: 1 I and/or anteiso-C 17: 1 B) and summed feature 3 (C 16: 1 Ɯ 7 c and/or C 16: 1 Ɯ 6 c) and anteiso-C 15: 0. Menaquinone-7 is the only respiratory quinone. The predominant polar lipids are phosphatidylethanolamine, three unidentified aminophospholipids and one unidentified phospholipid. The genomic DNA G+C content of strain HMF3095 T is 58.9 mol%.

The type strain is HMF3095T (=KCTC 52398T=NBRC 112669 T), isolated from freshwater of an artificial lake in Yong-in, Republic of Korea.