Enterocytozoon bieneusi

Zhong, Yalin, Zhou, Ziyao, Deng, Lei, Liu, Haifeng, Zhong, Zhijun, Ma, Xiaoping, Zhang, Kun, Wang, Yingzhu, Fu, Hualin & Peng, Guangneng, 2021, Prevalence and new genotypes of Enterocytozoon bieneusi in sheltered dogs and cats in Sichuan province, southwestern China, Parasite (Paris, France) 28 (31), pp. 1-7 : 3

Genetic characterizations of E. bieneusi

A total of five genotypes were identified in stray dogs in the present study by sequence analysis of the nucleotide sequences of the ITS region of E. bieneusi , including three known genotypes (CD9, PtEb IX, and Type IV) and two novel genotypes named SCD-1 and SCD-2 ( Table 1). Genotype CD9 was the most prevalent (92/136, 67.6%) and was observed in samples from all the three cities except for Guangyuan, followed by PtEb IX (30.1%, 41/136), which was detected in specimens from four locations. Genotypes Type IV, SCD-1, and SCD-2 were only found in one specimen, respectively (0.7%, 1/136).

Similarly, four genotypes were identified (CD9, Type IV, D, and PtEb IX) based on the analysis of the ITS sequences of the E. bieneusi -positive samples from stray cats ( Table 2). Genotype CD9 was predominant, accounting for 50% (11/ 22). This was followed by genotype Type IV (27.3%, 6/22), which was detected in both Chengdu and Ya’ an cities. Genotype D (18.2%, 4/22) was detected in both Chengdu and Ya’ an cities. Genotype PtEb IX was only found in one specimen in Chengdu city (4.5%, 1/22).