Fukomys darlingi (Thomas, 1895)

Šumbera, R., Uhrová, M., Begall, S., Caspar, K. R., Jerkovičová, D., Daele, P. Van, Chitaukali, W. N., Faulkes, C. G., Bennett, N. C., Johannes, C., Burda, H. & Mikula, O., 2023, The biology of an isolated Mashona mole-rat population from southern Malawi, with implications for the diversity and biogeography of the genus Fukomys, Organisms Diversity & Evolution (New York, N. Y.) 23 (3), pp. 603-620 : 607

Karyotype of F. darlingi from Nsanje

The karyotype of F. darlingi from the Nsanje population was established from a blood sample taken from one male kept at the University of Duisburg-Essen (F2 offspring of wild-caught mole-rats). Lymphocytes in whole blood cultures were stimulated to enter the cell cycle using phytohemagglutinin L (PAN Biotech, 3 μg/mL) for 2 days at 37 ° C. Mitotic cells were arrested using colcemid (Ciba, 80 ng / mL). Preparation and Giemsa staining followed standard protocols. Chromosomes in C-metaphases were examined. Altogether, 12 mitotic cells were analysed .