Polypheretima kalimpaaensis Fahri & Amaliah, 2017

Fahri, Amaliah, Rizki, Annawaty & Nguyen, Anh D., 2017, The earthworm genus Polypheretima Michaelsen, 1934 (Annelida: Clitellata: Megascolecidae) from Sulawesi, Indonesia, with descriptions of four new species, Raffles Bulletin of Zoology 65, pp. 559-573 : 564-567

Polypheretima kalimpaaensis Fahri & Amaliah , new species

( Fig. 5 )

Material examined. Holotype. Clitellate ( MZB Oli. 0051), Kalimpa’a Lake , Secondary Forest, Lore Lindu National Park (01°19′33″S, 120°18′29.6″E), elevation of 1,593 m asl., Central Sulawesi Province, Indonesia, 5 June 2016, leg. Fahri, Rizki Amaliah, Sahlan, Auni Ade Putri.

Paratypes. 5 clitellates ( MZB Oli. 0053), same data as for holotype ; 5 clitellates ( MZB Oli. 0052), same locality as for holotype, 31 October 2016, leg. Fahri, Rizki Amaliah, Rika Hafriani H, Evans Muhdiyono, Adhi Pranata, Ardiansyah, Sahlan ; 10 clitellates ( UNTAD Oli. 0004), same locality as for holotype, 31 October 2016, leg. Fahri, Rizki Amaliah, Rika Hafriani H, Evans Muhdiyono, Adhi Pranata, Ardiansyah, Sahlan.

Other material. 15 clitellates ( UNTAD Oli. 0005), same locality as for holotype, 31 October 2016, leg. Fahri, Rizki Amaliah, Rika Hafriani H, Evans Muhdiyono, Adhi Pranata, Ardiansyah, Sahlan.

Diagnosis. Medium size, length 124–156 mm, diameter 3–5 mm, segments 150–223. Prostomium prolobous. First dorsal pore in 12/13. Setae 58–64 in v, 42–74 in vii, 69–85 in viii, 48–52 in xxv, and 8–10 between male porophores in xviii. Spermathecae arranged in two pairs of pores in 5/6/7, about 3–7 per battery. Genital markings large, on setal rings, paired (rarely unpaired) in xix-xx, sometimes xxi. Holandric.

Etymology. The new species is named after Lake Kalimpa’a, in Lore Lindu National Park, where the type material was found.

Description. External characters. Body generally cylindrical. Medium size, length 124–156 mm, diameter 3–5 mm, segments 150–223. For living specimens, pre-clitellum and post-clitellum somewhat purplish pink, and clitellum brownish pink; for preserved specimens, colouration paler.

Prostomium prolobous. First dorsal pore in 12/13. Setae 58–64 in v, 42–74 in vii, 69–85 in viii, 48–52 in xxv, and 8–10 between male porophores in xviii; setae distance aa=1–2ab, zz=1–2zy. The number of setae in vii variable in specimens ( Table 4). Clitellum annular, xiv–xvi, smooth without setae and dorsal pores. Female pore single in mid-ventral xiv.

Spermathecal pores large, paired lateroventrally in 5/6/7. Ventral distance between spermathecal pores about 0.35× body circumference. Genital markings absent in the spermathecal region.

Male pores located deeply inside copulatory pouches in xviii; each copulatory pouch with a large, crescentic opening. Distance between the openings of copulatory pouches about 0.2× body circumference ventrally. Genital markings large, on setal rings, paired (rarely unpaired) in xix–xx ( Table 4). Internal characters. Septa 3/4/5/6/7/8 thick, 8/9/10 absent, 10/11/12/13 thin. Gizzard large, round after viii. Last hearts in xiii. Intestinal origin at xv; caeca absent. Pharyngeal micronephridia well-developed on septum 4/5. Typhlosole simple, lamelliform. Lymph glands absent.

Spermathecae small, 16–20 altogether in vi–vii: 8–10 in each of segment vi and vii (3–7 in each side of segment vi and vii, respectively). The number of spermathecae variable in specimens ( Table 4). Ampulla more or less subcylindrial; duct very short. Diverticulum cylindrical, much shorter than ampulla, slightly expanded at distal end; stalk attached to duct at base.

Holandric. Testes sacs paired in x and xi, small, separated, ventral, yellowish. Seminal vesicles in xi and xii. Ovaries paired in xiii and ovisacs developing in xiii. Prostate gland racemose, paired in xvi–xx. Prostatic ducts C-shaped. No accessory glands.

Habitat and ecology. Specimens were found in secondary forests near Kalimpa’a Lake at 1,593 m asl. They live just under rotten woods in sandy soils, and we did not find them in other habitats (sandy soils without rotting woods near the lake, and sandy soils with rich litters but not rotting woods). Besides worms, several beetle larvae were also found. We also found its cocoons in the collecting area. Cocoons are white, oval-shaped with a diameter of 4 mm.

Remarks. Polypheretima kalimpaaensis , new species, is keyed to the P. elongata species group. The species is fairly similar to almost all species in the elongata group, except P. phacellotheca and P. sahlani , in having spermathecal pores in 5/6/7 ( Table 6). However, the species is clearly different from its congeners in having 3–7 spermathecae in each battery and 42–74 setae in vii.

Figures

Fig. 5. Polypheretima kalimpaaensis sp. nov., Holotype. A, Spermathecal pores; B, Spermathecae (amp=ampulla, dv=diverticulum); C, Cocoon; D, Male pore region, ventral view (mp=opening of copulatory pouch, gm=genital marking); E, Male pore region, lateral view; F, Prostate gland. Scale bar = 1 mm (for A, B, D, E, F), 1 cm (for C).