Pseudonannolene granulata, Iniesta & Bouzan & Brescovit, 2023

Iniesta, Luiz Felipe Moretti, Bouzan, Rodrigo Salvador & Brescovit, Antonio Domingos, 2023, A reassessment of the Neotropical genus Pseudonannolene Silvestri, 1895: cladistic analysis, biogeography, and taxonomic review (Spirostreptida: Pseudonannolenidae), European Journal of Taxonomy 867 (1), pp. 1-312 : 105-106

Pseudonannolene granulata sp. nov.

urn:lsid:zoobank.org:act:6AE0ABDC-08C5-47E4-A941-A3DE4234297C

Figs 18C–D , 26B, D–E , 27 , 153–154 , 175A , 179J , 190 ; Suppl. file 4: Figs 198B, 200D, 202E

Diagnosis

Pseudonannolene granulata sp. nov. resembles P. buhrnheimi by having metazonites granulated ( Figs 26B,D , 27 , 153 , 200D) and epiproct with triangular process ( Figs 153B , 202E). Males of P.granulata differ by having stipes of gnathochilarium with proximal projections bearing setae ( Figs 175A , 198B).

Etymology

The species epithet is derived from the Latin adjective ‘ granulatus ’; in reference to the granular striations on metazonite of the species.

Material examined

Holotype BRAZIL • ♂; Rio de Janeiro, Cambuci, Balneário Santa Inês ; [-21.541444, -41.931761]; 29 Dec. 2017; L. Ázara, M. Medrano and A.B. Kury leg.; MNRJ.

Paratypes (total: 4 ♂♂, 2 ♀♀, 3 immatures) BRAZIL • 1 ♀; same collection data as for holotype; MNRJ • 4 ♂♂, 1 ♀, 3 immatures; same collection data as for holotype; MNRJ .

Description

MEASUREMENTS. 53–55 body rings (1–2 apodous + telson). Males: body length 43.8–64.7 mm; maximum midbody diameter 3.2–4.5 mm. Females: body length 56.5–72.2 mm; maximum midbody diameter 4–5.4 mm.

COLOR. Body color brownish; head, antennae, and collum darker; prozonites anteriorly greyish; metazonites with a medial band darker and a posterior reddish; legs brownish.

HEAD. Antennae short, just reaching back to end of ring 5 when extended dorsally; relative antennomere lengths 1<2<3>4>5=6>7. Mandibular cardo with ventral margin narrow. Stipes of gnathochilarium with basal projections bearing setae. Ommatidial cluster well-developed, elliptical; ca 40 ommatidia in 5 rows.

BODY RINGS. Collum with lateral lobes broadly rounded, with ca 6 deep striae, strongly curved ectad ( Fig. 153A ). Well demarcated constriction between prozonite and metazonite ( Figs 26B , 27A , 153 , 200D); prozonites smooth; metazonites densely granulated and laterally with transverse striae above ozopore ( Figs 26B , 27A , 200D). Anterior sterna in midbody rings subrectangular, without transverse striae ( Fig. 175A ). Epiproct with a long triangular process ( Figs 153B , 202E).

FIRST LEG-PAIR OF MALES. Coxae (cx) short (less than half of remaining podomere lengths), subtriangular, with the base arched, densely setose ( Fig. 154A ); prefemoral process (prf) short (less than half of prefemur), subcylindrical, with long setae up to its median region ( Fig. 154B ); remaining podomeres with setae along the mesal region.

SECOND LEG-PAIR OF MALES. Coxa (cx) large and rounded; penis (pn) located at proximal region, rounded, not extended basally ( Fig. 154C ); prefemur compressed dorsoventrally; remaining podomeres setose.

GONOPODS. Gonocoxa (gcx) elongated, almost twice as long as telopodite, with the base arched; antero-posteriorly slightly flattened ( Fig. 154D–F ); with rows of papillae mesally. Seminal groove (sg) curved; arising medially on mesal cavity and terminating apically on the seminal apophysis (sa); protruded on squamous region of sl ( Fig. 154D ). Shoulder (sh) long, subtriangular. Telopodite (tp) almost as wide as gcx, with deep depression separating from sh and laterad projection ( Fig. 154D ); solenomere (sl) thin, with apicomesal process (amp) subtriangular; ectal process (ep) short, subtriangular, separating from amp by shallow notch; sa located at mesal portion, slightly curved ectad, visible apically. Internal branch (ib) subtriangular, narrow, surrounding basally tp as a shield; ib with setae along its entire margin exceeding apically seminal region of sl ( Fig. 154D–F ).

VULVAE. As typical for the genus. Bursa square-shaped, glabrous ( Fig. 179J ); internal and external valvae square-shaped, not acuminated apically; operculum narrow, slightly curved ectad.

Distribution

Known only from the type locality Cambuci, Rio de Janeiro State, Brazil ( Fig. 190 ).

Figures

Fig. 18. Living specimens. A. Pseudonannolene urbica Schubart, 1945, from Mogi das Cruzes, SP, Brazil. B. Pseudonannolene spp. (troglophilic and troglomorphic species) from Lassance, MG, Brazil. C–D. P.granulatasp. nov., from Cambuci, RJ, Brazil.E. P. spelaeaIniesta & Ferreira, 2013, from Carajás, PA, Brazil. F. Pseudonannolene spp., from Adrianópolis, PR, Brazil. Photos (A–B) by L.F.M. Iniesta; (C–D) courtesy of A.B. Kury, (E) courtesy of M.P. Oliveira, and (F) courtesy of C.A.R. Souza.

Fig. 26. SEM images of midbody rings. A. Pseudonannolene robsoni Iniesta & Ferreira, 2014 (IBSP 3506). B. P. granulata sp. nov. (MNRJ). C. Detail of the transverse striae of P. robsoni (IBSP 3506). D. Midbody ring in dorsal view of P. granulata sp. nov. (MNRJ). E. Ozospore of P. granulata sp. nov. (MNRJ). Scale bars: A–B, D = 1 mm; C = 0.1 mm; E = 0.02 mm.

Fig. 27. SEM images of midbody rings of Pseudonannolene granulata sp. nov. (MNRJ). A. Lateral view. B. Detail of the ozadene. C. Midbody ring in transversal view. Scale bars: A, C = 1 mm; B = 0.5 mm.

Fig. 153. Pseudonannolene granulata sp. nov., holotype, ♂ (MNRJ), in lateral view. A. Anterior region. B. Posterior region. Scale bars = 1 mm.

Fig. 154. Pseudonannolene granulata sp. nov., paratype, ♂ (MNRJ). A. First leg-pair. B. Detail of prefemur. C. Second leg-pair. D. Detail of telopodites, in anal view. E. Gonopods, in anal view. F. Gonopods, in oral view. Abbreviations: see Material and methods. Scale bars: A, C, E–F = 0.5 mm; B, D = 0.2 mm.

Fig. 175. Stereoscopic images of gnathochilarium and anterior sternum of species of Pseudonannolene Silvestri, 1895. A. P. granulata sp. nov. B. P. alata sp. nov. C. P. curvata sp. nov. D. P. aurea sp. nov. E. P. alegrensis. Images not to scale.

Fig. 179. Stereoscopic images of left vulva of species of Pseudonannolene Silvestri, 1895, in oral view. A. P. strinatii Mauriès, 1974. B. P. tocaiensis Fontanetti, 1996. C. P. tricolor Brölemann, 1902. D. P. typica Silvestri, 1895. E. P. xavieri Iniesta & Ferreira, 2014. F. P. bucculenta sp. nov. G. P. morettii sp. nov. H. P. insularis sp. nov. I. P. nicolau sp. nov. J. P. granulata sp. nov. K. P. alata sp. nov. L. P. curvata sp. nov. M. P. aurea sp. nov. Images not to scale.

Fig. 190. Distribution map of the speciesPseudonannolene alata sp. nov.,P. aureasp. nov., P. bucculenta sp. nov., P. curvata sp. nov., and P. granulata sp. nov.