Mycobacterium tuberculosis, (Zopf, 1883) Lehmann & Neumann

Li, Da-Wei, Deng, Xiao-Peng, He, Xin, Han, Xiu-Yan, Ma, Yu-Fang, Huang, Hui-Lian, Yu, Zhen-Long, Feng, Lei, Wang, Chao & Ma, Xiao-Chi, 2021, Eupholides A H, abietane diterpenoids from the roots of Euphorbia fischeriana, and their bioactivities, Phytochemistry (112593) 183, pp. 1-8 : 7

4.6. Inhibitory effects on Mycobacterium tuberculosis

The inhibitory effects of isolated diterpenoids against M. tuberculosis were investigated using an Alamar blue cell viability assay (Thermo Fisher Scientific Inc.) in 96-well microplates. The test materials were dissolved in DMSO at an initial concentration of 50 mM. Ethambutol was used as the positive control antimicrobial agent. M. tuberculosis and the tested diterpenoids were coincubated in culture medium at 37 ◦ C. Then, the bacterial cells were determined by absorbance measurement at 595 nm. The detailed procedure about M. tuberculosis inhibitory assay was performed as previous work ( Han et al., 2019).