Jania sagittata (J.V.Lamouroux) Blainville

Macagnan, Leonardo B., Venturin, Claudia S., Azevedo, Marina L., Harvey, Adela & Gurgel, C. Frederico D., 2023, Molecular systematics of Jania species (Corallinales, Rhodophyta) from south-eastern Australia based on cox 1 and psbA DNA sequence analyses, Phytotaxa 606 (2), pp. 87-103 : 99

Jania sagittata (J.V.Lamouroux) Blainville

— Jania sagittata is considered a worldwide distributed species ( Johansen and Silva 1978, Guiry and Guiry 2022). Its typical morphology has been reported in temperate Australia including Tasmania, Brazil, Indonesia, Kenya, Mauritius, New Zealand, Philippines, Somalia, and South Africa ( Farr et al. 2009, Harvey et al. 2020). However, according to Harvey et al. (2020), ‘Most published records and geographicdistribution statements for J. sagittata require verification by voucher specimen examination’. The cox 1 and psb A results here agree with published molecular data ( Hind and Saunders 2013) that also sequenced and identified Australian specimens morphologically identified as stereotypical J. sagittata in Australia and New Zealand. However, the topotype or type material of J. sagittata from Mauritius has not been analyzed to conclusively confirm the correct application of this name in other parts of the world.

All of the phylogenetic and SDM analyses reported here for cox 1 and psb A markers were consistent in recognizing that all south-eastern Australian and New Zealand sequenced specimens of J. sagittata correspond to a single species ( Figs 1–4 ). In cox 1 phylogenies ( Fig. 1 ), J. sagittata was sister to J. cultrata (Harvey) Kim, Guiry and Choi from South Africa and differed by a 5.8% sequence divergence. Psb A phylogenies showed that Australia and New Zealand share the same J. sagittata species with 0.81% intraspecific sequence divergence ( Fig. 3 ).

Figures

FIGURE 1. Maximum likelihood phylogenetic tree of Jania species (Rhodophyta) based on cox1 gene with focus on specimens from south-eastern Australia. Numbers above branches represent non-parametric bootstrap support (values <70 omitted). Tree tip names are composed by species name, GenBank accession number and specimen locality (for sequences downloaded from GenBank), or species name, herbarium code for all sequenced specimens presented by that sequence, and specimen locality (for sequences produced in this study, all marked in bold). Scale bar = substitutions per site.

FIGURE 2. Bayesian phylogenetic tree of Jania species (Rhodophyta) based on cox1 gene with focus on specimens from south-eastern Australia. Numbers above branches represent posterior probabilities (values <0.5 were omitted). Some branches were shortened to fit the figure. Specimens sequenced in this study marked in bold and have their herbarium code identified. Colored columns indicate species delimitation methods results: ABGD; ASAP P (p-value partition), ASAP W (w rank partition); GMYC S (single-threshold), GMYC M (multiple-threshold); PTP B (Bayesian inference), PTP M (Heuristic or Maximum Likelihood); and SPN 95% and 99% of parsimony probability limit. Black column indicates the consensus across all species delimitation results. Numbers below to each column indicate the total number of species partitions for each method or the consensus. Jania squamata in the PTP B result was divided into three different species.

FIGURE 3. Maximum likelihood phylogeny of Jania species based on psbA gene focusing on specimens from south-eastern Australia. Numbers above branches represent non-parametric bootstrap support (values <70 omitted). Species names are followed by GenBank accession number and specimen locality (for sequences downloaded from GenBank), or species name, herbarium codes for all specimens sharing that particular haplotype, and specimen locality (marked in bold for sequences produced in this study). Scale bar = substitutions per site.

FIGURE 4. Bayesian phylogenetic tree of Jania species based on psbA gene with focus on specimens from south-eastern Australia. Numbers above branches represent posterior probabilities (values <0.5 omitted). Specimens sequenced in this study are marked in bold and have their herbarium code identified. Colored columns indicate species delimitation results: ABGD; ASAP P (p-value partition), ASAP W (w rank partition); GMYC S (single-threshold), GMYC M (multiple-threshold); PTP B (Bayesian inference), PTP M (Heuristic or Maximum Likelihood); and SPN 95% and 99% of parsimony probability limit. Black column indicates the consensus across all species delimitation results. Numbers below each column indicate the total number of species partitions for each method, including the consensus.