Allium sativum

Khan, W., Khatoon, N., Arshad, S., Mohammed, O. B., Ullah, S., Ullah, I., Romman, M., Parvez, R. & Mahmoud, A. H., 2022, Evaluation of vegetables grown in dry mountainous regions for soil transmitted helminths contamination, Brazilian Journal of Biology (e 238953) 82, pp. 1-10 : 3

Twenty five different type of vegetables including: (1) Allium sativum (garlic) (2) Capsicum annum (coriander) (3) Brassica compastris (mustard) (4) Lactuca sativa (lettuce salad) (5) Mentha longifolia (mint) (6) Daucus carota (carrot) (7) Allium cepa (onion) (8) Spinacia oleracea (spinach) (9) Raphanus raphanistrum (raddish) (10) Zingiber officinale (ginger) (11) Solanu tuberosum (potato) (12) Brassica olerace (cabbage) (13) Capsium annum (Chilli) (14) Brassica rapa (turnip) (15) Pisum sativam (peas) (16) Lycopersicon esculentum (Tomato) (17) Colocasia esculenta (taro) (18) Abelmoschus esculantum (Lady finger) (19) Momordica charanjtia (bitter gourd) (20) Solanum melongena (Brinjal) (21) Praecitrullus fistulosus (round gourd) (22) Capsicum annum (green pepper) (23) Cucumis sativus (Cucumber) (24) Luffa cylindrica (vegetable sponge) and (25) Brassica oleracea (cauliflower) were purchased from the selected vegetable markets in all the four districts.A questionnaire comprised on the questions such as educational status of the vendors, type of vegetables, means of display,washed before display was used. In this study fifty vendors participated.

2.3. Sample processing for parasites

The collected specimens of vegetables were placed in isolated plastic bags.Each of the bags containing vegetable samples were labeled with a unique number with date of collection. All the samples were brought to the Parasitology Laboratory, Department of Zoology, University of Malakand for screening of parasites. Of about 200 grams of each specimen of vegetables was kept for atleast 15 minutes in physiological solution of one liter in volume. After that the samples were shaken gently with the help of mechanical shaker for upto 20 minutes.After sedimentation about 10 millilitres of the sediment was separated in to a centrifuge tube. The centrifuge tube was centrifuged at 3000 rpm as to get concentrated the parasitic stages (ova, larave and cysts) for about five minutes. After completion of centrifugation, the supernatant was decanted and the sediment was agitated slightly by hand to redistribute the parasitic stages. Finally, the sediment was examined under a light microscope [Labomed 400] using × 10 and × 40 objectives.