Myxobolus rasmusseni, Tilley & Barry & Hanington & Goater, 2024

Tilley, Molly F., Barry, Danielle, Hanington, Patrick C. & Goater, Cameron P., 2024, Description, life cycle, and development of the myxozoan Myxobolus rasmusseni n. sp. in fathead minnows, Pimephales promelas: A possible emerging pathogen in southern Alberta, Canada, International Journal for Parasitology: Parasites and Wildlife 24, pp. 100944-100944 : 100944-

3.4. Evidence of the Myxobolus rasmusseni n. sp. life cycle

18S rDNA gene sequences amplified from the 10 TAM-releasing oligochaete worms corresponded to two species of annelid in the family Tubificidae . Sequences from 8 of the 10 worms were 96–99% similar to known sequences of Ilyodrilus templetoni , and 2 of the 10 were 100% similar to Tubifex. Both myxozoan DNA sequences were compared against the sequence generated for M. rasmusseni n. sp. and to other sequences available in GenBank. The myxozoan 18S rDNA gene sequences (PP591956) recovered from the two T. tubifex worms were a 100% match with the consensus sequence of M. rasmusseni n. sp. myxospores from fathead minnows. The closest match (93–94%) in 18S rDNA sequences (PP951958) recovered from triactinomyxon spores released from each of the 8 I. templetoni worms were to Dicauda athernoidi ( Loch et al., 2017) , a myxozoan of the head region of emerald and mimic shiners in North America.