Hepatozoon

Xiao, Xiao, Qi, Rui, Han, Hui-Ju, Liu, Jian-Wei, Qin, Xiang-Rong, Fang, Li-Zhu, Zhou, Chuan-Min, Gong, Xiao-Qing, Lei, Si-Cong & Yu, Xue-Jie, 2019, Molecular identification and phylogenetic analysis of Cryptosporidium, Hepatozoon and Spirometra in snakes from central China, International Journal for Parasitology: Parasites and Wildlife 10, pp. 274-280 : 276

3.2. Occurrence of Hepatozoon

Hepatozoon DNA was detected in blood samples of L. rufozonatus and G. brevicaudus by nested PCR and sequencing. Four 18S rRNA DNA sequences obtained in this study were 100% identical to each other, and were 99.4% identical to corresponding regions of H. ayorgbor isolated from the house snake ( Lamprophis fuliginosus, EF 157822) in Ghana, and 98.51% identical to H. ayorgbor isolated from the greater bandicoot rat ( Bandicota indica, AB 181504) in Chiang Mai, Thailand ( Fig. 2 ). Sequences analysis showed a 529bp fragment of the representative sequence had 100% similarity with partial SSU sequence from the king rat snake (KF939627) in Shanghai.

Figures

Fig. 2. Maximum likelihood phylogenetic tree based on the 18S rRNA gene of Hepatozoon. The phylogenetic tree was constructed with the 18S rRNA gene sequences (670bp) by using the General time reversible model with MEGA 7.0; the bootstrap values were calculated with 1000 replicates. Representative sequences of Hepatozoon detected in this study are in bold print and marked by circles. Scale bar indicates nucleotide substitutions per site.