Paramoebidium hamatum Bench & M.M. White, Mycologia

Barón, Daniel E. & Valle, Laia Guàrdia, 2018, First record of trichomycetes associated with aquatic insects from Colombian Moorland and Andean forests, Phytotaxa 361 (1), pp. 1-24 : 11

Paramoebidium hamatum Bench & M.M. White, Mycologia 104: 303. 2012. ( Figs 35–39 )

Specimens examined: — COLOMBIA. SANTANDER:Tona; Río El Roble, prepared from Leptohyphes sp. nymphs, D. Baron, COL5–Tr11; California; Río San Francisco, prepared from Camelobaetidius sp. nymphs, D. Baron, COL8–Tr21; Suratá; Río Suratá, prepared from Camelobaetidius sp. nymphs, D. Baron, COL9–Tr17; Suratá; Río Suratá, prepared from Leptohyphes sp. nymphs, D. Baron, COL9–Tr18; Piedecuesta; Río El Rasgón, prepared from Tricorythodes sp. nymphs, D. Baron, COL10–Tr27 and Tr28; Piedecuesta; Río El Rasgón, prepared from Camelobaetidius sp. nymphs, D. Baron, COL10–Tr30; Piedecuesta; Río El Rasgón, prepared from Mayobaetis sp. nymphs, D. Baron, COL11–Tr34, Tr35 and Tr37; Piedecuesta; Río Rasgón, prepared from Leptohyphes sp. nymphs, D. Baron, COL12–Tr38; Tona; Río Dos Aguas, prepared from Mayobaetis sp. nymphs, D. Baron, COL18–Tr54.

Comments: — P. hamatum has been reported from Idaho, USA, in both Chironomidae and Ephemerptera hosts ( Bench & White 2012). It was also reported from Italy in the mayfly Baetis alpinus (Valle et al. 2014) . Our specimens were found attached to the hindgut lining of Baetidae and Leptohpyphidae. Thalli measuring 65–1163 μm long, with a horseshoe shaped holdfast measuring 2.1–10.5 μm diam. Thallus widened at the basal area 13.5–16 μm diameter. Ellipsoidal cystospores measuring 32 × 6 μm. This is the first record of a Paramoebidium species within Leptohyphidae (Panamerican family of myflies). In fact, only one harpellid species, Allantomyces zopilotei , from Mexico, was previously reported associated with the genera Trichorythodes and Leptohyphes (Valle et al. 2008) , Both Camelobaetidius (reported above associated with P. santanderensis ) and Mayobaetis ( Baetidae ) are new hosts for Amoedidiales.

Smittium culicis Tuzet & Manier ex Kobayasi , Bulletin of the National Science Museum Tokyo 12: 322. 1970. (Figs 40–42)

Specimens examined: — COLOMBIA. SANTANDER: Tona; Río Agua Blanca, prepared from Chironomidae sp. Larvae, D. Baron, COL1-Tr2; California; Río San Francisco, prepared from Chironomidae sp. Larvae, D. Baron, COL8-Tr23; Berlin; Río Cardal, prepared from Chironomidae larvae, D. Baron, COL15-Tr47, Tr48.

Comments: — Smittium culicis is a cosmopolitan species and has been reported from nearly all continents where Dipteran host have been dissected for trichomycete research, counting France ( Manier 1970; Tuzet & Manier 1947), Spain ( Valle & Santamaria 2004), Italy ( Valle et al. 2013), Tunisia ( Manier et al. 1964), Canada, New Zealand and USA ( Lichtwardt 1986), Chile ( Lichtwardt & Arenas 1996), Argentina ( Lichtwardt et al. 1999) and now Colombia. Our specimens have a branched thallus with a holdfast measuring 5.3–12 μm diameter and trichospore measuring (16–)18–22 × 3.6–7.3 μm, with a collar of 6.2–13 μm long. Zygospores not found.

Figures

FIGURES 35–39. Paramoebidium hamatum from Baetidae and Tricorythidae. 35. Thallus overview. 36. Terminal papilla (arrow). 37. Detail of the holdfast in thallus attached to the hindgut lining. 38. Mature thallus and ellipsoidal cytospora in the initial attachment phase (arrow). Bar = 50 μm. 39. Thallus overview attached with other thalli. Bar = 25 μm.