Hemicycliophora undefined-3

Subbotin, Sergei A., Chitambar, John J., Chizhov, Vlamidir N., Stanley, Jason D., Inserra, Renato N., Doucet, Marcelo E., Clure, Michael M, Ye, Weimin, Yeates, George W., Mollov, Dimitre S., Cantalapiedra-Navarrete, Carolina, Vovlas, Nicola, Berg, Esther Van Den & Castillo, Pablo, 2014, Molecular phylogeny, diagnostics, and diversity of plant-parasitic nematodes of the genus Hemicycliophora (Nematoda: Hemicycliophoridae), Zoological Journal of the Linnean Society 171 (3), pp. 475-506 : 491-492

HEMICYCLIOPHORA SP. 3

( FIGS 1B, H, N , 3J; TABLES 1, S7)

This species is characterized by a ventrally arcuate body; sheath closely adpressed to body, but detached over postvulval body area, lateral fields marked by breaks and anastomoses occasionally with a faint, short, and irregular longitudinal line, annuli outside lateral fields marked with numerous more or less distinct fine longitudinal scratches or ridges, sometimes a few distinct short, irregular lines mark cuticle near lateral fields in the midbody to vulval region, lip region rounded anteriorly with three annuli, labial disc dome-shaped, elevated, vulval lips modified, about one annulus long, tail more or less conical, more so in posterior quarter, usually gradually tapering to a finely rounded or acute terminus; sometimes this portion is slightly offset by a greater dorsal curvature. No males were found. This species was found only in Arizona, USA.

This species resembles H. raskii but differs from it mainly by a protruded labial disc (vs. not protruded) and shorter stylet length (63–71 vs. 80–94 μm) and smaller RVan (number of annuli between vulva and anus) (5–8 vs. 9–14). This species is clearly different from H. raskii in the ITS and D2-D3 of 28S rRNA gene sequences.

Figures

Figure 1. Photomicrographs of specimens of populations of selected Hemicycliophora species. A–F, anterior region; G–L, lateral field; M–R, posterior region. A, G, M, Hemicycliophora conida (Washington State, USA); B, H, N, Hemicycliophora sp. 3 (Arizona, USA); C, I, O, Hemicycliophora sp. 8 (California, USA); D, J, P, Hemicycliophora raskii (California, USA); E, K, Q, Hemicycliophora sp. 10 (California, USA); F, L, R, Hemicycliophora californica (California, USA). Scale bars: A–F, M–R = 10 μm; G–L = 5 μm.

Figure 3. Scanning electron microscope (SEM) micrographs of specimens of populations of selected Hemicycliophora species. A–D, lip region; E–L, lateral field. A, Hemicycliophora wyei (North Carolina, USA) (CD683); B, Hemicycliophora poranga (California, USA) (CD714); C, Hemicycliophora sp. 3 (Arizona, USA) (CD715); D, Hemicycliophora californica (California, USA) (CD826B); E, Hemicycliophora gracilis (California, USA) (CD45); F, H. wyei (North Carolina, USA) (CD679); G, H. californica (CD826B); H, H. wyei (CD683); I, H. poranga (CD714); J, Hemicycliophora sp. 3 (CD715); K, Hemicycliophora sp. 4 (North Carolina, USA) (CD675); L, H. californica CD826B). Scale bars: A–C, E, G–J, L = 5 μm; D = 2 μm; F, K = 10 μm.