Trypanosoma spp

Qiu, Yongjin, Kajihara, Masahiro, Harima, Hayato, Hang'ombe, Bernard Mudenda, Nakao, Ryo, Hayashida, Kyoko, Mori-Kajihara, Akina, Dr., Changula, Katendi, Eto, Yoshiki, Ndebe, Joseph, Yoshida, Reiko, Takadate, Yoshihiro, Dr., Mwizabi, Daniel, Kawabata, Hiroki, Simuunza, Martin, Mweene, Aaron, Sawa, Hirofumi, Takada, Ayato, Professor & Sugimoto, Chihiro, 2019, Molecular characterization and phylogenetic analysis of Trypanosoma spp. detected from striped leaf-nosed bats (Hipposideros vittatus) in Zambia, International Journal for Parasitology: Parasites and Wildlife 9, pp. 234-238 : 235-236

3.2. Sequence analysis of Trypanosoma spp .

Sequence analysis of gGAPDH revealed the presence of two distinct sequence types. The gGAPDH sequence type 1 from the samples ZB17- 105, −111, and −115 (GenBank no. LC415422) showed 97.0% (555/ 572 bp) identity with the T. conorhini isolate TCC2156, which was detected from a triatomine ( Triatomia rubrofasciata ) of Hawaii (GenBank no. MF144731) ( Espinosa-Álvarez et al., 2018). The sequence type 2 from the samples ZB17-108 and −109 (GenBank no. LC415423) showed 94.2% (539/572 bp) identity with the T. dionisii isolate TryCC495 from a bat ( Carollia perspicillata ) in Brazil (GenBank no. GQ140363) ( Cavazzana et al., 2010).

The same grouping was obtained by the sequence analysis of 18S rRNA. The 18S rRNA gene of the sequence type 1 from the samples ZB17-105, −111, and −115 (GenBank no. LC415424) showed 97.7% (1074/1099 bp) identity with Trypanosoma sp. isolate NanDoum1 from the African palm civet (N. binotata) in Cameroon (GenBank no. FM202492) ( Hamilton et al., 2009). The sequence type 2 from the samples ZB17-108 and −109 (GenBank no. LC415425) showed 95.2% (1033/1084 bp) identity with the T. dionisii isolate P3 from a bat ( Pipistrellus pipistrellus ) captured in the United Kingdom (GenBank no. AJ009151) ( Stevens et al., 1998).