Dioszegia milinica Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 89

Dioszegia milinica Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828759. Fig. 8B .

Etymology: the specific epithet milinica refers to the geographic origin of the type strain, Milin county, Tibet.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are ovoid and ellipsoidal, 2.9– 6.4 × 5.0–10.3 μm and single, budding is polar ( Fig. 8B ), a sediment is formed. After 1 mo at 17 °C, a ring and sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is orange, butyrous, smooth and glossy. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are not produced.

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, galactose, sucrose, maltose, cellobiose, trehalose, melibiose, raffinose, melezitose, inulin, soluble starch (delayed and weak), D-xylose, L-arabinose, D-arabinose, L-rhamnose (delayed and weak), D-glucosamine (delayed and weak), galactitol, D-glucitol, succinate and citrate are assimilated as sole carbon sources. L-sorbose, lactose, D-ribose, N-Acetyl-D-glucosamine, methanol, ethanol, glycerol, erythritol, ribitol, Dmannitol, Methyl-α- D-glucoside, salicin, DL-lactate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate (weak), L-lysine (weak) and ethylamine hydrochloride are assimilated as sole nitrogen sources. Sodium nitrite and cadaverine dihydrochloride are not assimilated. Maximum growth temperature is 23 °C. Growth in vitamin-free medium is positive. Starch-like substances are produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Di. milinica differs from the closely related species Di. aurantiaca in its inability to assimilate D-ribose, N- Acetyl-D-glucosamine, glycerol, erythritol, ribitol, D-mannitol, Methyl-α- D-glucoside, salicin, DL-lactate and sodium nitrite and its ability to assimilate inulin and ethylamine ( Table S1.9 ).

Typus: China, Milin county, Tibet, obtained from a leaf of an unidentified plant, Sep. 2015, Q.-M. Wang (holotype CGMCC 2.5628 T preserved in a metabolically inactive state, ex-type CBS 15563 = GPS21.3B8).

Figures

Fig. 8. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A) Sa. pseudoflava CGMCC 2.5811T; (B) Di. milinica CGMCC 2.5628T; (C, D) Di. heilongjiangensis CGMCC 2.5674T; (E, F) Di. ovata CGMCC 2.3625T; (G, H) Di. maotaiensis CGMCC 2.4537T; (I) Di. kandeliae CGMCC 2.5658T; (J) Bu. phyllostachydis CGMCC 2.5812T; (K, L) Bu. cremeum CGMCC 2.4427T; (M, N) Bu. pseudopanici CGMCC 2.4024T; (O, P) Bu. phyllophilum CGMCC 2.3320T. Bars = 10 μm.