Dioszegia heilongjiangensis Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 89

Dioszegia heilongjiangensis Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828760. Fig. 8C, D .

Etymology: the specific epithet heilongjiangensis refers to the geographic origin of the type strain, Heilongjiang province.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are subglobosal and ellipsoidal, 3.2– 5.0 × 4.5– 7.3 μm and single, budding is polar ( Fig. 8C ), a sediment is formed. After 1 mo at 17 °C, a ring and sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is yellowish to light orange, butyrous, smooth and partly wrinkled. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are subglobosal to napiform, 4.0– 5.0 × 5.0– 6.0 μm ( Fig. 8D ).

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, galactose, sucrose (weak), maltose (weak), cellobiose (weak), trehalose (weak), melibiose, raffinose, melezitose, inulin (weak), D-xylose (delayed), L-arabinose, Darabinose (delayed and weak), galactitol (weak), D-glucitol, salicin (weak) and succinate are assimilated as sole carbon sources. L-sorbose, lactose, soluble starch, D-ribose, L-rhamnose, D-glucosamine, N-Acetyl-D-glucosamine, methanol, ethanol, glycerol, erythritol, ribitol, D-mannitol, Methyl-α- Dglucoside, DL-lactate, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate, L-lysine and cadaverine dihydrochloride are assimilated as sole nitrogen sources. Potassium nitrate, sodium nitrite and ethylamine hydrochloride are not assimilated. Maximum growth temperature is 26– 27 °C. Growth in vitamin-free medium is negative. Starch-like substances are produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Di. heilongjiangensis differs from the closely related species Di. changbaiensis and Di. cryoxerica in its inability to assimilate D-ribose, L-rhamnose and D-mannitol and its ability to grow in vitamin-free medium ( Table S1.9 ).

Typus: China, Chelu county, Heilongjiang province, obtained from a leaf of an unidentified plant, Aug. 2014, Q.-M. Wang (holotype CGMCC 2.5674 T preserved in a metabolically inactive state, ex-type CBS 13957 = HLJ13.24).

Figures

Fig. 8. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A) Sa. pseudoflava CGMCC 2.5811T; (B) Di. milinica CGMCC 2.5628T; (C, D) Di. heilongjiangensis CGMCC 2.5674T; (E, F) Di. ovata CGMCC 2.3625T; (G, H) Di. maotaiensis CGMCC 2.4537T; (I) Di. kandeliae CGMCC 2.5658T; (J) Bu. phyllostachydis CGMCC 2.5812T; (K, L) Bu. cremeum CGMCC 2.4427T; (M, N) Bu. pseudopanici CGMCC 2.4024T; (O, P) Bu. phyllophilum CGMCC 2.3320T. Bars = 10 μm.