Variovorax humicola, Nguyen & Kim, 2016

Maeng, Soohyun, Kim, Ju-Young, Jang, Jun Hwee & Kim, Myung-Suk Kang and Myung Kyum, 2018, A report of 11 unrecorded bacterial species in Korea isolated in 2017, Journal of Species Research 7 (2), pp. 135-150 : 148

Description of Variovorax humicola 17U4-2

Cells are Gram-stain-positive and short rod-shaped. Colonies are yellow-colored after 3 days of incubation on R2A at 25°C. In the BIOLOG GEN III, D-maltose, D-trehalose, D-cellobiolose, gentiobiose, sucrose, D-turanose, stachyose, D-raffinose, D-melibiose, β -D-glucoside, D-salicin, neuraminic acid, α -D-glucose, D-fructose, D-mannitol, glycerol, L-histidine, D-gluconic acid, and L-malic acid were utilized as sole carbon source. But dextrin, α -D-lactose, N -glucosamine, β -mannosamin, N -galactosamin, D-mannose, D-galactose, 6-methyl-glucose, D-fucose, L-fucose, L-rhamnose, inosine, D-sorbitol, D-arabitol, myo-inositol, D-glucose, D-fructose, D-aspartic acid, D-serine, gelatin, glycyl-L-proline, L-alanine, L-arginine, L-aspartic acid, L-glutamic acid, pyroglutamic acid, L-serine, pectin, galacturonic acid, L-galactonic lactone, D-glucuronic acid, glucuronamide, mucic acid, quinic acid, D-saccharic acid, phenylacetic acid, methyl pyruvate, D-lactic acid, L-lactic acid, citric acid, α -glutaric acid, D-malic acid, succinic acid, tween 40, γ -butryric acid, α -butryric acid, β -butryric acid, acetoacetic acid, propionic acid, acetic acid, and formic acid were not utilized. In sensitivity tests, the tetrazolium redox dye is reduced at pH 6 but not at pH 5; the dye is reduced in the presence of 1% NaCl, 4% NaCl, sodium lactate, D-serine, tetrazolium violet, nalidixic acid, lithium chloride, potassium tellurite, aztreonam, and sodium butyrate; but not in the presence of 8% NaCl, fusidic acid, troleandomycin, rifamycin SV, minocycline, lincomycin, guanidine HCl, niaproof 4, vancomycin, tetrazolium blue, and sodium bromate.

In API 20NE system, positive for of reduction of nitrates (NO 3) to nitrogen (N 2), arginine dihydrolase, urease, esculin hydrolysis, β -galactosidase, D-glucose, Larabinose, D-mannose, D-mannitol, N -acetyl-D-glucosamine, D-maltose, potassium gluconate, adipic acid, malic acid, trisodium citrate, and phenylacetic acid. Negative for reduction of nitrates (NO 3) to nitrite (NO 2 -), indole production on tryptophan, glucose fermentation, gelatin hydrolysis, and capric acid.

In API 32GN system, positive for L-rhamnose, N -acetyl-glucosamine, D-ribose, D-saccharose (sucrose), D-maltose, suberic acid, sodium acetate, lactic acid, Lalanine, potassium 5-ketogluconate, 3-hydroxybenzoic acid, D-mannitol, D-glucose, salicin, D-melibiose, D-sorbitol, L-arabinose, propionic acid, valeric acid, L-histidine, potassium 2-ketogluconate, 3-hydroxybutyric acid, 4-hydroxybenzoic acid, and L-proline. Negative for inositol, itaconic acid, sodium malonate, glycogen, L-serine, D-fucose, capric acid, and trisodium citrate. Strain 17U4-2 (= NIBRBAC000500500) was isolated from a soil sample.