Steinernema afriCanUm, Machado & Bhat & Abolafia & Shokoohi & Fallet & Turlings & Tarasco & Půža & Kajuga & Yan & Toepfer, 2022

Machado, Ricardo A. R., Bhat, Aashaq Hussain, Abolafia, Joaquín, Shokoohi, Ebrahim, Fallet, Patrick, Turlings, Ted C. J., Tarasco, Eustachio, Půža, Vladimír, Kajuga, Joelle, Yan, Xun & Toepfer, Stefan, 2022, Steinernema africanum n. sp. (Rhabditida, Steinernematidae), a New Entomopathogenic Nematode Species Isolated in the Republic of Rwanda, Journal of Nematology 54 (1), pp. 1-28 : 5

Steinernema afriCanUm n. sp.

( Figures 1–7 and Tables 1–4)

Figures

Figure 1: Line drawings of Steinernema afriCanUm n. sp. IJ. (A) Stoma and pharynx region. (B) Anterior end. (C) Entire IJ. (D) Posterior end. IJ, infective juvenile.

Figure 2: Line drawings of first- and second-generation Steinernema afriCanUm n. sp. females. (A–E) First-generation female: (A) Stoma and pharynx region; (B) Lip region and stoma; (C) Vagina region; (D) Posterior end; (E) Entire female. (F–I) Second-generation female: (F) Stoma and pharynx region; (G) Lip region and stoma; (H) Vagina region; (I) Posterior end.

Figure 3: Line drawings of first- and second-generation Steinernema afriCanUm n. sp. males. (A–D) First-generation male: (A) Stoma and pharynx region; (B) Lip region and stoma; (C) Entire male; (D) Posterior end. (E–H) Second-generation male: (E) Stoma and pharynx region; (F) Lip region and stoma; (G) Entire male; (H) Posterior end.

Figure 4: Light microscope micrographs of first-generation adults and IJ of Steinernema afriCanUm n. sp. (A, B) Stoma and pharynx region of female and male, respectively. (C–E) Lip region and stoma of female, male and IJ, respectively. (F) Vagina region. (G–I) Entire female, male and IJ, respectively. (J) Posterior end of a female. (K, L) Posterior end of males. Pictures are in right lateral view. IJ, infective juvenile.

Figure 5: Light microscope micrographs of second-generation adults and IJ of Steinernema afriCanUm n. sp. (A–C) Stoma and pharynx region of female, male and IJ, respectively. Black arrow pointing the EP, white arrow pointing the bacteria sac. (D) Vagina region. (E, F) Entire female and male, respectively. (G, H) IJ bacterial sac and lateral field, respectively. (I–K) Posterior end of female, male, and IJ, respectively. Pictures are in right lateral view. EP, excretory pore; IJ, infective juvenile.

Figure 6: Scanning electron microscope micrographs of first-generation adults of Steinernema afriCanUm n. sp. (A, B) Lip region of a female and a male, respectively, in lateral view. Arrows pointing the amphids (C: cheilostom, G: gymnostom, S: stegostom, cp: cephalic papillae, and lp: labial papillae). (C) Male posterior end in sub-ventral view – GP, MP, ph. (D, E) Lip region of a female and a male, respectively, in frontal view. (F, G) EP of a female and a male, respectively. (H) Vulva. (I) Male posterior end in sub-ventral view – GP, MP, ph. (J) Male posterior end in sub-ventral view – GP, MP. (K) Female anus. Arrow pointing a post-anal pore. (L, M) Female tail in lateral and ventral views, respectively. Black arrow pointing a post-anal pore, white arrows pointing the phasmids. EP, excretory pore; GP, genital papillae; MP, mid-ventral papilla; ph, phasmid.

Figure 7: Scanning electron microscope micrographs of second-generation adults and IJ of Steinernema afriCanUm n. sp. (A–C) Lip region of female, male, and IJ in lateral view, respectively. Arrows pointing the amphids (C: cheilostom, G: gymnostom, S: stegostom, cp: cephalic papillae, lp: labial papillae). (D–F) Lip region of female, male, and IJ in frontal view, respectively. Black arrows point to the amphids, white arrow points to the dorsal gland opening. (G) EP of female. (H) Male posterior region in lateral view – GP, MP, ph. (I) EP of IJ. (J) Vulva. (K) IJ lateral field. (L, M) Female tail (arrows pointing the phasmids). (N) Male posterior region in lateral view. (O, P) IJ tail in lateral and ventral views, respectively. Arrows pointing the phasmids. EP, excretory pore; GP, genital papillae; MP, mid-ventral papilla; ph, phasmid.