Tupiella speciosa Darienko et Pröschold, 2017

Darienko, Tatyana & Pröschold, Thomas, 2017, Toward a monograph of non-marine Ulvophyceae using an integrative approach (Molecular phylogeny and systematics of terrestrial Ulvophyceae II.), Phytotaxa 324 (1), pp. 1-41 : 33

Tupiella speciosa Darienko et Pröschold sp. nov. ( Fig. 22A–K )

Diagnosis: The thallus from the attached prostrate and short, erect filaments. Branching is rare and occurs on one or both sides. The terminal cells of erected filaments are often tapered or slightly pointed (13.5 × 6.0–17.5 × 4.0 μm). Vegetative cells of young filaments are cylindrical with parietal chloroplasts containing a pyrenoid surrounded by two large starch grains and two large vacuoles, (8.5 × 4.0–12.5 × 4.0 μm). Vegetative cells are unicellular. The prostrate filaments occur in the form of 8–16-cell packages. The filaments are easy disintegrated in unicells or 2–4-celled packages in old cultures and are 10–14.2 μm in diameter. Reproduction by zoospores is not observed. Mature culture start to produce large swollen cells, which swell to approximately 20 μm in diameter, with chloroplasts, pyrenoids, and a strongly vacuolized cytoplasm; they possess a thick-layered cell wall without special ornamentation. The swollen cells start to grow up on one side up to long, thin filaments that become later branched or produce 2–4-cell packages. From those packages, short filaments (one per each cell) start to grow.

Type locality: Lednice n. M., Czech Republic.

Holotype (designated herein): The strain CCALA 423 is permanently cryopreserved in a metabolically inactive state (cryopreserved in liquid nitrogen) in the CCALA ( Culture Collection of Autotrophic Organisms ), Institute of Botany CAS, Czech Republic.

Iconotype (designated herein in support of the holotype): Fig. 22 in this study.

ITS-2 DNA Barcode: TUP 1 in Figs S2.

Figures

FIGURE 22. Morphology of Tupiella speciosa CCALA 423 grown on 3NBBM medium.