Hercospora tiliae (Pers.) Tul. & C. Tul., Select.

Norphanphoun, Chada, Hongsanan, Sinang, Doilom, Mingkwan, Bhat, Darbhe J., Wen, Ting-Chi, Senanayake, Indunil C., Bulgakov, Timur S. & Hyde, Kevin D., 2016, Lamproconiaceae fam. nov. to accommodate Lamproconium desmazieri, Phytotaxa 270 (2), pp. 89-102 : 98-100

Hercospora tiliae (Pers.) Tul. & C. Tul., Select. fung. carpol. (Paris) 2: 154 (1863) Figs. 5–6

Possible synonyms (See Index Fungorum 2016)

Facesoffungi number: FoF02452

Saprobic on branches and twigs of Tilia sp. Sexual morph: Stromata 700–800 μm wide, prosenchymatous around perithecia, delimited externally by greenish-blackened dense pseudoparenchymatous zone, interior whitish, composed of interwoven hyphae mixed with substrate cells, 3–5 ascomata in a stromata. Ascomata 1–1.05 mm high, 0.24–0.34 mm diam., (x = 1.02 × 334 μm, n = 10), perithecial, small, aggregated, scattered, globose to subglobose, light brown to dark brown, coriaceous, ostiolate, papillate. Papilla 625–645 μm high, 190–290 μm diam., (x = 640 × 250 μm, n = 10), converging and erumpent through stroma surface as single, large opening, wide at the top, narrowing towards the base, dark brown region around base of papilla. Peridium 10–20 μm wide (x = 16 μm, n = 10), comprises light brown, compressed, cells of textura angularis. Asci 140–175 μm × 17–24 μm diam., (x = 160 × 21 μm, n = 10), 8- spored, unitunicate, cylindrical, short-stalked, J- apical apparatus. Ascospores 20–25 μm × 9–11 μm diam., (x = 23 × 10 μm, n = 10), uniseriate, broadly ellipsoid, 1-septate, not or lightly constricted at the septa, hyaline, smooth. Asexual morph: Stromata prosenchymatous. Conidiomata pycnidial, uniloculate, ostiolate, ostiole surrounded by a superficial cap of sterile tissues. Conidiophores elongate. Conidia 14–16.5 × 4.5–6.5 μm (x = 15 × 5 μm, n = 10), hyaline ovoid to ellipsoid, one-celled.

Material examined:— SWEDEN. Uppland: Upl. Stockholm: Roslagstull Stockholm, on bark of Tilia sp. , L.

Romell, 1 April 1887, F 148711 ( S).

Notes:— Hercospora Fr. comprises 15 species ( Index Fungorum 2016) with Hercospora tiliae as the type. The characters of the genus include eustromatic, immersed, subepidermal, dark blackish brown, separate, uniloculate, multiloculate or convoluted and thick-walled conidiomata; conidiophores branched extensively at the base, less so above, hyaline, septate, smooth, often developing in mucilage, formed at the base and sides of the conidiomatal wall; and ellipsoid, thick-walled, hyaline, aseptate conidia (18–20 × 6–7.5 μm) ( Petrak 1938, Sutton 1980).

Phylogenetic studies ( Castlebury et al. 2002, Rossman et al. 2007, Voglmayr et al. 2012, Voglmayr & Jaklitsch 2014) based on LSU sequence data, placed H. tiliae in Diaporthales , genera incertae sedis, where it grouped with Melanconis desmazierii . In the present study based on maximum likelihood, maximum parsimony and Bayesian analyses of combined ITS and LSU sequence data, Lamproconium desmazierii (= Melanconis desmazieri ) and Hercospora tiliae , clustered in Lamproconiaceae fam. nov.