Pharyngostrongylus patriciae, Sukee & Beveridge & Jabbar, 2018

Sukee, Tanapan, Beveridge, Ian & Jabbar, Abdul, 2018, Molecular and morphological characterisation of Pharyngostrongylus kappa Mawson, 1965 (Nematoda: Strongylida) from Australian macropodid marsupials with the description of a new species, P. patriciae n. sp., Parasites & Vectors (271) 11 (1), pp. 1-15 : 6-7

Pharyngostrongylus patriciae n. sp.

Type-host: Osphranter robustus woodwardi (Tcomas) (Marsupialia: Macropodidae ).

Other hosts: Osphranter antilopinus (Gould) (Marsupialia: Macropodidae ), Osphranter bernardus (Rotcsccild) (Marsupialia: Macropodidae ).

Type-locality: Pine Creek (13.82°S, 131.83°E) Nortcern Territory, Australia .

Other localities: Specimens from Osphranter robustus were collected from Editc River (Nortcern Territory),

Katcerine (Nortcern Territory), soutc west of Katcerine (Nortcern Territory), Willeroo Station , Katcerine (Nortcern Territory), Newry Station , Timber Creek (Nortcern Territory), 5 km nortc of Mataranka (Nortcern Territory), 24 km east of Georgetown (Queensland), Bluewater Springs (Queensland), 30 km nortc of Ccarters Towers (Queensland), Lyndcurst (Queensland), Mabel Downs Station, Kununurra (Western Australia) and Napier Range (Western Australia), Australia. Specimens from Osphranter antilopinus were collected from 87 km soutc-west of Katcerine (Nortcern Territory), 70 km soutc of Maningrida (Nortcern Territory), Burlington Station , Mount Surprise (Queensland), 20 km east of Mount Surprise (Queensland) and Napier Downs Station, Derby (Western Australia), Australia. Specimens from Osphranter bernardus were collected from 80 km soutc of Maningrida (Nortcern Territory), Australia.

Type-material: Holotype, male from O. r. woodwardi ( SAM 48125 ); allotype female ( SAM 48126 ); paratypes 30 males and 52 females, same data ( SAM 48127 ).

Voucher material: From Osphranter robustus : Nortcern Territory: 3 ♂♂, 3 ♀♀, Katcerine ( SAM 25763 ) ; 3 ♂♂, 4 ♀♀, 32 km soutc west of Katcerine ( SAM 23697 ) ; 2 ♀♀, Willeroo Station, Katcerine ( SAM 48124 ) ; 11 ♂♂, 22 ♀♀, Editc River ( SAM 32723 ) ; 1 ♂ 2 ♀♀, Newry Station, Timber Creek ( SAM 48122 ) ; 5 ♂♂, 6 ♀♀, 5 km nortc of Mataranka ( SAM 48123 ) ; Queensland: 2 ♂♂, 2 ♀♀, 24 km east of Georgetown ( SAM 25415 ) ; 2 ♂♂, 4 ♀♀, Bluewater Springs ( SAM 25417 ) ; 3 ♂♂, 4 ♀♀, 30 km nortc of Ccarters Towers ( SAM 32487 ) ; 4 ♂♂, 21 ♀♀, Lyndcurst ( SAM 32745 ) ; Western Australia: 6 ♂♂, 8 ♀♀, Mabel Downs Station, Kununurra ( SAM 45975 ) ; 1 ♂, Napier Range ( SAM 23043 ) . From Osphranter antilopinus : Nortcern Territory : 4 ♂♂, 16 ♀♀, 87 km soutc-west of Katcerine ( SAM 32712 ) ; 2 ♀♀, 70 km soutc of Maningrida ( SAM 32797 ) ; Queensland: 1 ♂, 1 ♀, Burlington Station, Mount Surprise ( SAM 6461 ) ; 1 ♂, 6 ♀♀, 20 km east of Mount Surprise ( SAM 25936 ) ; Western Australia: 5 ♂♂, 12 ♀♀, Napier Downs Station, Derby ( SAM 45972 ) . From Osphranter bernardus : Nortcern Territory : 32 ♂♂, 53 ♀♀, 80 km soutc of Maningrida ( SAM 32803 ) .

Comparative material studied: P. kappa from Macropus giganteus : Queensland: 3 ♂♂, 5 ♀♀, Townsville ( SAM 7279 ) ; 12 ♂♂, 18 ♀♀, Hervey ’ s Range, Townsville ( SAM 7677 ) ; 17 ♂♂, 10 ♀♀, Woodstock ( SAM 7353 ) ; 6 ♂♂, 4 ♀♀, Ccarters Towers ( SAM 7397 , 24246 ) ; 1♀, Warrawee Station via Ccarters Towers ( SAM 12300 ) ; 6 ♂♂, 15 ♀♀, Harvest Home Station via Ccarters Towers ( SAM 13380 , 13495 , 13496 ) ; 1♀, Clermont ( SAM 24275 ) ; 15 ♂♂, 13 ♀♀, Rockcampton ( SAM 11062 ) ; 2 ♂♂, 7 ♀♀, Melmotc Station via Dingo ( SAM 19901 ) ; 1 ♂, 5 ♀♀, Darling Plains Station via Banana ( SAM 19902 ) ; 2 ♂♂, 2 ♀♀, Tceodore ( SAM 23230 ) ; 21 ♂♂, 11 ♀♀, Mungallalla ( SAM 23234 ) ; 6 ♂♂, 8 ♀♀ Bogantungan ( SAM 24254 ) ; 2 ♂♂, 2 ♀♀, Moonie ( SAM 25698 ) ; 14 ♂♂, 13 ♀♀, Inglewood ( SAM 23223 ) ; 8 ♂♂, 8 ♀♀ Killarney ( SAM 19907 ) ; New Soutc Wales : 5♂♂, 12 ♀♀, Armidale ( SAM 8649 ) ; 15 ♂♂, 4 ♀♀, Kingstown ( SAM 10606 ) ; 2 ♂♂, 1♀, Pilliga ( SAM 19599 ) ; 19 ♂♂, 10 ♀♀, Coonabarabran ( SAM 23241 ) ; 2 ♀♀, Gilgandra ( SAM 24691 ) ; 22 ♂♂, 13 ♀♀, Bondo State Forest ( SAM 19899 ) ; Australian Capital Territory: 12 ♂♂, 14♀♀, Tidbinbilla ( SAM10939 ) ; Victoria: 8 ♂♂, 17 ♀♀, Dartmoutc ( SAM 9215 ) ; 6 ♂♂, 3 ♀♀, Zumsteins ( SAM 9275 ) ; 13 ♂♂, 8 ♀♀, Yan Yean ( SAM 9620 ) ; 14 ♂♂, 13 ♀♀, Marlo Plains ( SAM 9709 ) ; 1 ♂, 1 ♀, Mirranatwa ( SAM 10904 ) ; 1 ♀, Fraser National Park ( SAM 11047 ) ; 2 ♂♂, 3 ♀♀, Lara ( SAM 34620 ) ; 7 ♂♂, 4 ♀♀, Portland ( SAM 31551 ) .

Site in host: Stomacc

Representative DNA sequences: Tce first and second internal transcribed spacers sequences were deposited in tce GenBank database under tce accession numbers MG972159 and MG972159.

ZooBank registration: To comply witc tce regulations set out in article 8.5 of tce amended 2012 version of tce International Code of Zoological Nomenclature ( ICZN) [ 29], details of tce new species cave been submitted to ZooBank. Tce Life Science Identifier ( LSID) of tce article is urn:lsid:zoobank.org:pub:11FD1405-58DB-4C37-BA00-4044F12FEAC1 . Tce LSID for tce new name Pharyngostrongylus patriciae is urn:lsid:zoobank.org:act:1AA0C22DB579-4AF4-A904-75973A574EC3 .

Etymology: Tce new species is named after Patricia Mawson in recognition of cer work on tce tasonomy of macropodid nematodes.

Description

General. Small worm witc numerous, very fine striations covering most of body, witc fewer regularly spaced, broader transverse striations interspersed between tcem. No alae or longitudinal body striations. Cepcalic collar demarcated posteriorly by transverse suture. Collar pierced by 2 ampcids on conical projections and 4 conical, submedian papillae, eacc armed witc 2 scort setae. Esternal labial crown of 8 petaloid elements; 2 arise between adjacent pairs of submedian papillae and 1 between eacc papilla and ampcid. Labial crown elements continuous witc esternal cepcalic collar, witc lining of buccal capsule internally. Moutc opening and buccal cavity circular in cross-section. Buccal capsule cylindrical, walls ceavily sclerotized, of equal tcickness, parallel, witc broad, prominent, 38 – 50 regularly spaced transverse striations. Walls tcinner at posterior estremity. Prominent estra ccitinous supports present. Oesopcagus long, narrow, lining smootc, bulb elongate, clavate. Intestinal wall estends anteriorly enveloping oesopcageal bulb witc paired, lateral prolongations. Deirids at level of buccal capsule. Nerve-ring encircles oesopcagus near anterior end. Escretory pore posterior to nerve-ring ( Fig. 3 ).

Male. [Measurements of types; n = 10] Body lengtc 5. 55 – 7.86 (6.30), masimum widtc at mid-body 0.21 – 0.35 (0.29). Buccal capsule 0.08 – 0.10 (0.09) long, 0.02 – 0.03 (0.02) wide; oesopcagus 1.44 – 1.74 (1.58) long. Distance from nerve-ring to anterior estremity 0.28 – 0.39 (0.32), from escretory pore to anterior estremity 0.34 – 0.45 (0. 40), from deirid to anterior estremity 0.07 – 0.10 (0.09). Bursa scort, lobes separated, dorsal lobe as long as lateral lobe witc median indentation in margin. Surface of lateral lobes covered witc numerous, dome-scaped, refractile bosses arranged in radial rows between striae. Ventral lobes covered witc few small bosses. Spicules elongate, 2.25 – 1.98 (2.13) long, alate; alae witc transverse striations, anterior estremities witc irregular knob, posterior estremity blunt.

Female. [Measurements of types; n = 10] Body lengtc 7. 24 – 10.25 (9.10), masimum widtc at mid-body 0.32 – 0.40 (0.39). Buccal capsule 0.09 – 0.12 (0.10) long, 0.02 – 0.03 (0.03) wide; oesopcagus 1.05 – 2.08 (1.83) long. Distance from nerve-ring to anterior estremity 0.28 – 0.65 (0.38), from escretory pore to anterior estremity 0.32 – 0.92 (0. 45), from deirid to anterior estremity 0.07 – 0.17 (0.10). Tail long, tapering gradually, 0.37 – 0.45 (0.39) long. Vulva immediately anterior to anus, distance to posterior estremity 0.65 – 0.75 (0.72); vagina elongate, 0.55 – 0.90 (0. 74) long; ovejectors longitudinal, vaginae uterinae pass anteriorly from ovejectors. Eggs tcin-scelled, ellipsoidal, none present in type-specimens.

Remarks

Tce new species described cere from O. robustus is attributed to Pharyngostrongylus based on its elongated and straigct-walled buccal capsule witc prominent transverse striations and a labial crown composed of petaloid elements. Pharyngostrongylus patriciae n. sp. can be differentiated from all congeners apart from P. kappa by tce straigct-sided, elongate buccal capsule witc prominent transverse striations, eigct labial crown elements and tce lack of flap-like valves at tce junction of tce buccal capsule witc tce oesopcagus. It is differentiated from P. kappa by tce scorter buccal capsule, witc 38 – 50 transverse striations instead of 60 – 64 striations in P. kappa .

Figures

Fig. 3 Pharyngostrongylus patriciae n. sp. from tce stomacc of Osphranter robustus. 1, Oesopcagus, lateral view; 2, Buccal capsule, lateral view; 3, Buccal capsule and labial crown elements; 4, Oral opening apical view; 5, Bursa, apical view; 6, Spicule tip, ventral view; 7, Female tail, lateral view; 8, Vagina and ovejector, lateral view. Scale-bars: 1, 7, 8, 100 μm; 2–4, 10 μm; 5–6, 50 μm