Culture media

Silva, E. R., Dall’Oglio, E. L., Vasconcelos, L. G. & Morais, E. B., 2022, Decolorization of the benzidine-based azo dye Congo red by the new strain Shewanella xiamenensis G 5 - 03, Brazilian Journal of Biology (e 237386) 82, pp. 1-7 : 2

2.2. Culture media and synthetic wastewater

Tryptic soy broth (TSB) used in bacterial inoculum production was purchased from Himedia Labs. Agar was added in TSB (1.5% w/v) to prepare a solid media used in stock cultures of the bacterial strain.

CR sterile stock solution was diluted in rich mineral medium, consisted of (g L- 1): K 2 HPO 4 1.6, KH 2 PO 4 0.2, (NH 4) 2 SO 4 1.0, MgSO 4 .7H 2 O 0.2, FeSO 4 .7H 2 O 0.01, NaCl 0.1, CaCl 2.H 2 O 0.02, glucose 3.0, and yeast extract 1.0 ( Franciscon et al., 2009), at required concentration to create the synthetic dye loaded wastewater .