Enterocytozoon bieneusi

Jian, Yingna, Zhang, Xueyong, Wang, Guanghua, Wang, Geping, Li, Xiuping, Ma, Liqing, Liang, Qin, Li, Chenglin & Zhang, Yong, 2023, First report on the molecular detection of Enterocytozoon bieneusi in livestock and wildlife around Qinghai Lake in the Qinghai-Tibetan Plateau area, China, International Journal for Parasitology: Parasites and Wildlife 21, pp. 110-115 : 111

2.2. Screening of E. bieneusi by nested-PCR

All DNA samples were screened by nested-PCR with the outer primers EBITS3/EBITS4 and inner primers EBITS1/EBITS2.4 for amplifying the 390 bp fragment of the ITS gene were conducted according to a previous study in 2002 ( Buckholt et al., 2002). PCR primers and cycling protocols to amplify target sequences were described in Supplementary Table 1. The secondary PCR products were tested by 1.5% agarose gel containing 1 × GelStain (TransGen Biotech, China), and visualized using a G: BOX F3 Gel Documentation System (Syngene, UK).