Pseudonocardia lutea, Gao & Piao & Wang & Shi & Guo & Song & Xiang & Zhao & Wang, 2018

Gao, Yuhang, Piao, Chenyu, Wang, Han, Shi, Linlin, Guo, Xiaowei, Song, Jia, Xiang, Wensheng, Zhao, Junwei & Wang, Xiangjing, 2018, Pseudonocardia lutea sp. nov., a novel actinobacterium isolated from soil in Chad, International Journal of Systematic and Evolutionary Microbiology 68 (6), pp. 1992-1997 : 1995-1996

DESCRIPTION OF PSEUDONOCARDIA LUTEA SP. NOV.

Pseudonocardia lutea (lu′ te.a. L. fem. adj. lutea yellow, being a yellow organism).

An aerobic, Gram-stain-positive actinobacterium that grows well and forms an extensively branched substrate and aerial mycelia. Chains of rod-shaped spores with smooth surfaces are produced on ISP 2 medium. The strain shows good growth on ISP 2, CA and BA media, moderate growth on ISP 1, ISP 3, ISP 4, ISP 6 and ISP 7 media, and poor growth on ISP 5 and NA. No diffusible pigment is produced. Grows at 10–45 Ǫ C (optimum, 28–37 Ǫ C), pH 5.0–10.0 (optimum, pH 6.0–7.0) and 0–10 % (w/v) NaCl (optimum, 1–3 %). Hydrolysis of cellulose and Tweens (20, 40 and 80) are positive. Hydrolysis of starch and aesculin, production of H 2 S and urease, milk coagulation and peptonization, nitrate reduction and liquefaction of gelatin are negative. D- Fructose, D- galactose, inositol, lactose, D- mannose, raffinose, L- rhamnose, D- ribose, D- sorbitol, sucrose and D- xylose are utilized as sole carbon sources, but L- arabinose, D- glucose, maltose and D- mannitol are not. L- Alanine, L- arginine, L- asparagine, L- aspartic acid, creatine, L- glutamic acid, L- glutamine, glycine, L- proline, L- serine and L- tyrosine are utilized as sole nitrogen sources, but L- threonine is not. The cell wall belongs to type IV. Whole-cell sugars contain glucose, arabinose, xylose and galactose. The major menaquinone is MK-8(H 4). The phospholipid profile consists of diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, hydroxyphosphatidylmethylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol mannosides, one unidentified glycolipid and one unidentified lipid. The major cellular fatty acids of strain NEAU-G57 T are C 16: 0 and C 16: 1 Ɯ 7 c.

The type strain is NEAU-G57 T (=JCM 32387 T =CGMCC 4.7397 T), isolated from a soil sample collected from the bottom of a river in Chad. The DNA G+C content of the type strain is 73.3 mol%.