Rhizoclonium minutissimum (Zeller) P. C. Silva

Ichihara, Kensuke, Shimada, Satoshi & Miyaji, Kazuyuki, 2016, Two new green algae, Rhizoclonium fractum sp. nov. and R. umbraticum sp. nov., from tropical and subtropical brackish waters of Japan, Phytotaxa 266 (4), pp. 231-249 : 246

Rhizoclonium minutissimum (Zeller) P. C. Silva ( Figs. 7 , 8 )

Basionym: Cladophora minutissima Zeller 1873 , Algae collected by S. Kurz in Arracan and British Burma, determined and systematically arranged by G. Zeller. Journal of the Asiatic Society of Bengal 42 (2): 175–193.

Type locality: Elephant Point, Pegu, Burma ( Silva et al. 1996: 785).

Figures

FIGURE 7. Field-collected specimens of Cladophoraceae sp. 2 (Rhizoclonium minutissimum). (A) Field-collected specimens. (B, C) Morphology of field-collected specimens. (D, E) Filamentous thalli with non-septate rhizoids. (F) Tip of filamentous thalli. (G–I) Spindle- and band-shaped chloroplasts. Arrowhead points to a pyrenoid. (J) Autofluorescence of chloroplasts. (K) Cells stained with DAPI, showing nuclei. (L) Cells stained with Lugol’s iodine, showing pyrenoids. (M, N) Pyrenoids observed by transmission electron microscopy. Pyrenoids have starch plates. (M) Three or two thylakoid membranes traversing a pyrenoid. (33) One thylakoid membrane. Scale bar = 5 mm (A); Scale bars = 40 μm (B, C); Scale bars = 20 μm (D–G, K, L); Scale bars = 10 μm (H–J); Scale bars = 0.5 μm (M); Scale bars = 1 μm (N).

FIGURE 8. Cultured material of Cladophoraceae sp. 2 (Rhizoclonium minutissimum). (A, B) Morphology of filamentous thalli. (C) Filamentous thalli with rhizoid-like structure. (D, E) Spindle- and band-shaped chloroplasts with pyrenoids (arrowheads). (F) Autofluorescence of chloroplasts. (G) Cells stained with DAPI, showing nuclei. (H) Cells stained with Lugol’s iodine, showing the pyrenoids. (I) Mature sporangia with spores and vegetative cells. Arrowhead points to a mature sporangium. (J) Detail of a hapteroid attachment cell. Scale bar = 20 μm (A–C, G–I); Scale bars = 10 μm (D–F, K).