Giardia agilis, Künstler, 1882

Lyu, Zhangxia, Cheng, Jiaoni, Shao, Jingru, Ye, Qingqing, Bai, Huixian & Wen, Jianfan, 2020, An investigation of the prevalence of Giardia agilis in anuran amphibians from fourteen areas in China, International Journal for Parasitology: Parasites and Wildlife 12, pp. 46-52 : 47

2.2. Morphological identi fi cation of G. agilis

All anuran amphibian samples were investigated. The trophozoite samples described above were dropped on slides. The slides were prepared with trophozoite supernatant in 0.65% sodium chloride solution and 20 mm square coverslips. All slides were checked before PCR and all coverslips were completely checked. All slides were examined under oil immersion by 40× and 100× HCX PL APO objectives on a Leica DM2500 microscope (Leica, Wetzlar, Germany). The images were captured by a Leica DFC450 C digital camera. Sample handling and photomicrographs of scanning electron microscope were processed at the Kunming Medical University (Kunming, China) using HITACHI 3700N (Tokyo, Japan).