Cellulomonas rhizosphaerae BT

Kim, Ju-Young, Maeng, Soohyun, Park, Yuna, Lee, Sang Eun, Han, Joo Hyun, Cha, In-Tae & Kim, Ki-eun Lee and Myung Kyum, 2021, Twelve unrecorded UV-resistant bacterial species isolated in 2020, Journal of Species Research 10 (4), pp. 321-335 : 328

Description of Cellulomonas rhizosphaerae BT 580

Cells are Gram-stain-positive, non-flagellate, and rod-shaped. Colonies are white colored after 3 days of incubation on R2 A at 25℃. In the BIOLOG GEN III test, positive for D-maltose, D-trehalose, D-cellobiolose, gentiobiose, sucrose, D-turanose, α -D-glucose, D-mannose, D-fructose, inosine, glycerol, lithium chloride, Tween 40, acetoacetic acid, propionic acid, acetic acid, formic acid and sodium butyrate; weak positive for dextrin, D-raffinose, α -D-lactose, D-melibiose, β -D-glucoside, D-salicin, N -glucosamine, β -mannosamin, 1% NaCl, Dgalactose, 6-methyl-glucose, fusidic acid, D-serine, D-sorbitol, D-mannitol, myo-inositol, D-gluose, D-serine, troleandomycin, rifamycin SV, minocycline, gelatin, L-arginine, L-aspartic acid, L-serine, lincomycin, Niaproof 4, pectin, galacturonic acid, galactonic lactone, D-gluconic acid, mucic acid, vancomycin, tetrazolium violet, tetrazolium blue, methyl pyruvate, D-lactic acid, α -glutaric acid, nalidixic acid, potassium tellurite and aztreonam; but negative for stachyose, pH 6, pH 5, N -galactosamin, neuraminic acid, 4% NaCl, 8% NaCl, D-fucose, L-fucose, D-galactose, sodium lactate, D-arabitol, D-fructose, D-aspartic acid, glyctl-L-proline, L-alanine, L-glutamic acid, L-histidine, pyroglutamic acid, guanidine HCl, D-glucuronic acid, glucuronamide, quinic acid, D-saccharic acid, pheylacetic acid, L-lactic acid, citric acid, D-malic acid, L-malic acid, succinic acid, γ -butryric acid, α -butryric acid, β -butryric acid, α -butyric acid and sodium bromate. In API 20NE, urease, esculin hydrolysis and β -galactosidase were utilized;whereas reduction of nitrates (NO 3 -) to nitrite (NO 2 -), reduction of nitrates (NO 3 -) to nitrogen (N 2), indole production on tryptophan, glucose fermentation, arginine dihydrolase, gelatin hydrolysis, D-glucose, L-arabinose, D-mannose, D-mannitol, N -acetyl-D-glucosamine, D-maltose, potassium gluconate, capric acid, adipic acid, malic acid, trisodium citrate and phenylacetic acid were not utilized. In the API 32GN test, L-rhamnose, N -acetyl-glucosamine, D-ribose, inositol, D-saccharose (sucrose), D-maltose, itaconic acid, suberic acid, sodium malonate, sodium acetate, lactic acid, L-alanine, potassium 5-ketogluconate, glycogen, 3-hydroxybenzoic acid, L-serine, D-mannitol, D-glucose, salicin, D-melibiose, D-fucose and D-sorbitol were utilized; while L-arabinose, propionic acid, capric acid, valeric acid, trisodium citrate, L-histidine, potassium 2-ketogluconate, 3-hydroxybutyric acid, 4-hydroxybenzoic acid and L-proline were not utilized. Strain BT 580 (= NIBRBAC000506208) was isolated from a soil sample from Guri-si, Gyeonggi-do, Korea .