Timaviella sp.

Mai, Truc, Johansen, Jeffrey R., Pietrasiak, Nicole, Bohunická, Markéta & Martin, Michael P., 2018, Revision of the Synechococcales (Cyanobacteria) through recognition of four families including Oculatellaceae fam. nov. and Trichocoleaceae fam. nov. and six new genera containing 14 species, Phytotaxa 365 (1), pp. 1-59 : 34

Timaviella sp. WMT-WP7-NPA

Description:— D1-D1’ helix 75 nucleotides long, with a 3’ basal unilateral bulge of 8 nucleotides (5’-CAUCCCAA-3’), having several internal loops at positions 14–17/50–54, 21–23/44–46 and 26–27/40–41. Terminal loop 4 nucleotides, 5’-GCAA-3’ ( Fig. 6j ). Box B helix 42 nucleotides long, with a basal internal loop at position 5/37–38, one unpaired adenine residue at position 9 and a U/C mismatch at position 13/29 ( Fig. 7i ). V2 helix 12 nucleotides long ( Fig. 8g ). V3 helix 61 nucleotides long, with three internal loops at positions 5/55–57, 10/48–50 and 23/34–35, separated from the terminal loop by a 5’-GU:AG-3’ clamp ( Fig. 9i ).

Compared to other species, D1’-D1’ helix structure has identical basal sequence (region 1–9/59–75) especially the 3’ side loop and a U/U mismatch at position 9/75 ( Fig. 6j ), and the V3 helix is almost identical in shape to T. obliquedivisa with minor differences in sequence. The strain is similar in the base of Box B helix to T. radians at position 1–5 and its complement on the 3’ strand ( Fig. 7i ); however, it differs in other molecular characteristics.

Collection locality:— White Mountain Wilderness, California, USA.

Taxonomic notes:— The reference material for this species did not survive in culture, and thus has no morphological description. The species has high similarity in the 16S rRNA sequence to other species of this genus ( Table 11), but we do not have enough morphological information to formally describe this as a species within Timaviella .

Figures

FIGURE 6. D1-D1’ stems of species described in Oculatellaceae.The stem structures of genera described previously in other publications (Oculatella, Thermoleptolyngbya, Timaviella) or in publications under provision (Trichotorquatus) are not shown here.

FIGURE 7. Box B helices of species described in Oculatellaceae. The structures of genera described previously in other publications (Oculatella, Thermoleptolyngbya, Timaviella) or in publications under provision (Trichotorquatus) are not shown here.

FIGURE 8. V2 helices of species described in Oculatellaceae. Several species do not have this structure, including Pegethrix convoluta, P. indistincta, Antartic Pegethrix species, Cartusia fontana¸ Kaiparowitsia implicata.

FIGURE 9. V3 helices of species described in Oculatellaceae. All species described have this structure. The V3 helix of Cartusia aeruginosa is missing because we do not have the full length of its ITS region.