Calcarisporium yuanyangense H. Yu, 2023

Zhu, Juye, Tang, Dexiang, Liu, Zuoheng, Luo, Lijun & Yu, Hong, 2023, Calcarisporium yuanyangense sp. nov. (Calcarisporiaceae, Hypocreales), a fungal pathogen isolated from Ophiocordyceps nutans, Phytotaxa 612 (3), pp. 272-282 : 278

Calcarisporium yuanyangense H. Yu bis & J.Y. Zhu, sp. nov. Fig. 2

MycoBank: MB 848860

Etymology:— The epithet “ yuanyangense ” referred to collection sites for this species, Yuanyang County.

Holotype:— Xinjie Town , Yuanyang County, Yunnan Province, China (23°04′42.98″N, 102°51′41.61″E, alt. 2053.70m), on an O. nutans , September 25, 2022, Hong Yu bis, YHH 2209025 , holotype; YFCC 22099256 , ex-type living culture.

Description:—Sexual morph: Sexual morphs were not found. Asexual morph: Colonies effuse, woolly-felty texture, yellowish-white, mycelium immersed, consisting of branched, septate, smooth, hyaline hyphae, hyphae 1.81– 2.89 μm wide. colonies on PDA reached 20–22 mm in diameter after 10 days at 25 ℃. Conidiophores 12.49–119.42 × 1.49–3.01 μm, erect, hyaline, verticillately branched, with 1–5 verticils, each verticil with 1–5 conidiogenous cells. Conidiogenous cells 12.31–51.84 × 1.40–3.08 μm, hyaline, swollen at base, gradually tapering near the apex, holoblastic to polyblastic, sympodial, apically with a cluster of conidium-bearing denticles. conidiogenous scars 0.86–1.78μm. Conidia 6.33–9.68 × 1.87–2.63 μm, unicellular, hyaline, smooth-walled, thin-walled, ovoid to ellipsoid, acuminate.

Know distribution:— Xinjie Town, Yuanyang County, Yunnan Province, China.

Note:— Calcarisporium yuanyangense was isolated from O. nutans and was different from the host reported in previous studies. Calcarisporium yuanyangense was identified as a unique species based on both morphological characteristics and phylogenetic analysis; both demonstrate that it was distinct from other recognized species in the genus Calcarisporium . Phylogenetic analysis based on five genes showed strong support (BP = 100% and PP = 100%) with being an independent branch. Calcarisporium yuanyangense and C. arbuscula were similar in conidia shape, but the conidia of C. yuanyangense (6.33–9.68 × 1.87–2.63 μm) were larger and had a higher length/width ratio than those of C. arbuscula ( Table 2 ).

Figures

FIGURE 2. Calcarisporium yuanyangense. a. Fungus on O. nutans. b. Surface (left) and abaxial surface (right) of the colony of C. yuanyangense on PDA medium. c–i. Conidiophores with conidia. m, n. Conidiophores without conidia. o, p. Conidiogenous scars. q, r. Conidia. Bars: a, b = 2cm, c, f, g, i, j = 20μm. d, e, h, k–r = 10μm.