Calycina shangrilana (W.Y. Zhuang & H.D. Zheng) Lestari & Chethana, 2022

Lestari, Anis S. & Thilini Chethana, K. W., 2022, Morpho-phylogenetic insights reveal Bisporella montana as Calycina montana comb. nov. (Pezizellaceae, Helotiales), Phytotaxa 558 (2), pp. 185-202 : 193

Calycina shangrilana (W.Y. Zhuang & H.D. Zheng) Lestari & Chethana , comb. nov. ( FIGURE 5 )

Basionym: Bisporella shangrilana W.Y. Zhuang & H.D. Zheng, Zhuang, Zheng & Ren, Mycosystema 36(4): 407 (2017)

Index Fungorum number: IF 570312, Facesoffungi number: FoF 05942

Holotype: HMAS 275568

Saprobic on woody substrate. Sexual morph: Apothecia 0.4–1.7 × 0.3–1.3 mm in dried state, gregarious, arising singly or in clusters, pinkish-orange, stipitate. Stipe 0.3–0.7 x 0.1–0.4 mm in dried condition, white to cream color different from the disc. Receptacle discoid, bright orange to yellow color. Margin cream color to hyaline. Disc concave. Ectal excipulum 33–59 µm (= 42.7 µm, n = 10) at flank near the stipe, composed of gelatinized-hyaline cells of textura intricata. Medullary excipulum 24–31 µm (= 27.1 µm, n = 10) at flank near the stipe, distinctively separate from ectal excipulum, composed of thin-walled, hyaline cells of textura oblita. Hymenium 109–140 µm (= 125.3 µm, n = 10). Paraphyses 1.4–1.9 µm in diam. (= 1.8 µm, n = 10) at the terminal, filiform, septate. Asci 119–146 × 5.4–8 µm (= 133 × 6.7 µm, n = 10), unitunicate, 8-spored, cylindrical, subclavate at the top, rounded apex, J+ (euamyloid), long substipitate base, arising from croziers. Ascospores 7.3–11 × 3.1–4.2 µm (= 8.3 × 3.8 µm, n = 10), ellipsoid, 0–1- septate, partially biseriate to uniseriate, hyaline, guttulate, rounded at both ends. Asexual morph: Undetermined.

Material examined: China, Yunnan, Shangrila, Bitahai , 12 August 2008, on rotten wood, X.Q. Zhang & D.Z. Ren, ( HMAS 275568 , holotype) .

Notes: This specimen is the type strain of Bisporella shangrilana ( HMAS 275568), synonymized under Calycina , as C. shangrilana in the current study. Calycina shangrilana is characterized by orange-pinkish apothecia, gelatinized textura intricata cells in the ectal excipulum, and a calycina-like apical ring, which fit with the generic concept of Calycina ( Baral & Marson 2005) . Based on ITS and LSU phylogenetic analyses, the type strain ( HMAS 275568) clusters with the other C. shangrilana strains within Calycina clade ( Figure 1 ).

Figures

FIGURE 1. The phylogram of combined ITS and LSU sequence data for genera in Pezizellaceae. Maximum likelihood bootstrap values greater than 70% and posterior probability values greater than 0.95 given near the nodes. The new geographical record highlighted in red and type strains are in bold. The tree is rooted to Chlorencoelia torta (JAC 14068, KUSF 52256 and ICMP 21732).

FIGURE 5. Calycina shangrilana (HMAS 275568, holotype). a A herbarium package b–c Apothecia on the woody substrate. d Cross sections of an apothecium. e Close up of hymenium at the margin. f Close up of ectal excipulum cells. g Filiform paraphyses (mounted in Congo red). h–j Asci (h, i mounted in Congo red, j mounted in Melzer’s agent). k Tip of the ascus (J+, in Melzer’s agent with KOH treatment). l Close up of croziers at the base of an immature ascus. m–n Ascospores (mounted in Congo red). Scale bars: b–c = 475 μm, d = 350 μm, e = 100 μm, f = 15 μm, g = 20 μm, h–j = 17 μm, k = 5 μm, l–n = 9 μm.