Streptomyces diacarni, Li & Wang & Zhou & Lin & Lu, 2019

Li, Lei, Wang, Jie, Zhou, Yong-jun, Lin, Hou-wen & Lu, Yan-hua, 2019, Streptomyces reniochalinae sp. nov. and Streptomyces diacarni sp. nov., from marine sponges, International Journal of Systematic and Evolutionary Microbiology 69 (1), pp. 99-104 : 103

DESCRIPTION OF STREPTOMYCES DIACARNI SP. NOV.

Streptomyces diacarni (di.a.car′ ni. N.L. gen. n. diacarni of the marine sponge Diacarnus megaspinorhabdosa).

Aerobic, Gram-stain-positive, non-motile, non-acid–alcohol-fast actinomycete that forms extensively branched substrate mycelia and white aerial hyphae which differentiated into chains of smooth-surfaced spores. Growths occurs at 20–40 Ǫ C and at pH 6.0–11.0 with 0–15 % NaCl. Growths occurs at 20–40 Ǫ C and at pH 6.0–11.0 with 0–15 % NaCl. Gelatin, and Tweens 20, 40 and 60 are degraded, but CMC and Tween 80 are not. Degradation of aesculin and starch are weakly positive. Milk coagulation, peptonization and nitrate reduction are positive. Utilizes L- arabinose, D- arabinose, cellobiose, D- fructose, D- galactose, D- glucose, glycerol, lactose, maltose, D- mannitol, D- mannose, melibiose, L-rhamnose, D- ribose, sodium acetate, sodium pyruvate, sodium citrate, trehalose and D- xylose as sole carbon sources; weakly utilizes inositol and raffinose, but not dulcitol, D- sorbitol, sucrose or D- xylitol. Utilizes L- alanine, L- arginine, L- asparagine, L- glutamine, glycine, L- histidine, L-hydroxyproline, L- lysine, L- proline, L- serine, L- threonine and xanthine as sole nitrogen sources; and weakly utilizes hypoxanthine, L- leucine and L- valine, but not adenine, L-aspartic acid, L- cystenine, L- glutamic acid, L- methionine, L-phenylalanine, L- tryptophan or L- tyrosine. The cell wall contains LL- diaminopimelic acid and whole-cell sugars are glucose, galactose and ribose. Diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol are the predominant polar lipids. The menaquinone system contains MK-9(H 6) as a major component, and MK-9(H 4) and MK-9(H 8) as minorities. The major fatty acids are iso-C 16: 0, anteiso-C 15: 0 and anteiso-C 17: 0. The G+C content is 72.1 mol% of the 7.6 Mb draft genome.

The type strain, LHW51701 T (=DSM 106126 T =CCTCC AA 2018017 T), was isolated from a Diacarnus megaspinorhabdosa marine sponge collected in the South China Sea. The GenBank accession number for the 16S rRNA gene sequence and the draft genome sequence of the type strain are KX347890 and QOIN00000000, respectively .