Neodeightonia arengae Y.R. Xiong, Manawas., K.D. Hyde & Z.Y. Dong, 2022

Xiong, Yinru, Manawasinghe, Ishara S., Liao, Chunfang, Hyde, Kevin D. & Dong, Zhangyong, 2022, Neodeightonia arengae sp. nov., Botryosphaeriaceous taxa on Arenga tremula (Arecaceae) from Guangdong, China, Phytotaxa 530 (2), pp. 130-140 : 136

Neodeightonia arengae Y.R. Xiong, Manawas., K.D. Hyde & Z.Y. Dong , sp. nov. ( FIGURE 2 )

Index Fungorum number: IF558659

Facesoffungi number: FoF 10227

Etymology: Epithet refers to the host genus from which the fungus was isolated

Holotype: ZHKU 21-0095

Saprobic on leaf of Arenga tremula (Arecaceae) . Sexual morph: not observed. Asexual morph: Conidiomata 200–330 µm length × 140–190 µm diam. (x =250 × 160 µm, n=10), pycnidial, semi-immersed, solitary, globose, unilocular, black dots, a crack on the surface of the host where the conidiomata is located. Peridium 20–30 µm (x =21 µm, n=5), composed of thick-walled, brown-black cells of textura angularis, thin inner wall, almost reduced to conidiogenesis region. Conidiophores reduced to conidogenous cells. Conidiogenous cells hyaline, holoblastic, rough. Conidia 10–30 µm × 10–15 µm (x =21 × 11 µm, n=40), hyaline, subglobose to ellipsoid, aseptate, granular content, one to two large guttules, broadly rounded at both ends, thin-wall without mucilaginous.

Culture characters: colonies on PDA reach 7 cm diam. at 28˚C after five days. Upper view wrinkled, filamentous, entire margin, flat, cloudy, fluffy for aerial hyphae, become gray-black with time, dense for aerial hyphae, reverse becomes black.

Material examined: CHINA. South China Botanical Garden, Guangzhou, Guangdong Province, dead leaves of Arenga tremula (Blanco) Becc. , 17 December 2020, Yinru Xiong , (holotype ZHKU 21-0095 ), living cultures ZHKUCC 21-0074 ex-holotype; ZHKUCC 21-0075, ZHKUCC 21-0076 ex-Paratype .

Notes: Three isolates in the present study clustered together with the Neodeightonia species. The taxa from this study form two sister clades to N. palmicola (MFLUCC 10-0822) and N. planchoniae (MFLUCC 17-2427). Neodeightonia planchoniae is a sexual morph and it was characterized by sheaths around the ascospores, while N. arenge is an asexual morph and there was no sheath observed around the conidia. Neodeightonia arengae differs from N. palmicola conidia by the absence of the truncate base, while N. palmicola conidia sometimes appeared truncate at the base ( Table 3 ). Neodeightonia arengae has one or two large guttules in conidia, while the conidia of N. palmicola lack guttules ( Liu et al. 2010). The conidia of N. arengae have one morphological stage, hyaline, and aseptate, while the conidia of N. palmicola have two morphological stages initially hyaline. With age, conidia become cinnamon to sepia, forming one septum. Based on these polyphasic approaches we identified our isolates as novel Neodeightonia species.

Figures

FIGURE 2. Neodeightonia arengae (ZHKU 21-0095 Holotype): a. Specimen observed. b–c. Conidiomata. d, e Peridium. f. Conidiogenous cells. g–j. conidia. k. germinating conidia. l. The front of culture on PDA at 25℃. m. The reverse of culture on PDA at 25℃. Scale bars: b, c = 300 µm, d = 100 µm, e = 30 µm, f = 15 µm, g–j, k = 10 µm.