Tripartiella bulbosa

Saha, Mandira & Bandyopadhyay, P. K., 2016, First report of some known Trichodinella epizootica Šramek-Hušek, 1953, and Tripartiella Lom, 1959 from the cultured Oranda Gold Fish (Carassius auratus auratus) in India, Zootaxa 4184 (1), pp. 130-140 : 134-136

Tripartiella bulbosa ( Davis 1947; Lom 1959)

( Fig. 2 ; Table 3)

Description (Number of specimens measured—9). Body disc shaped measuring 18.0–23.4 (20.8±2.1), Adhesive discs dark and with 13.2–18.7 (16.7±3.8) diameter. Uniquely shaped denticles with denticular ring about 6.4–9.3 (7.8±2.0).The blade is elongated, slanted obliquely backwards with parallel lateral margins, which are constructed at either end. ( Fig. 2 A, 2B) The distal margins of the blades are almost rounded. Tangent point is flat and it is situated lower than the distal margin with a broader membrane width of 1.7–2.7 (2.2±0.7). Anterior projection of the blade is becoming reduced to an insignificant tubercle. The well developed posterior hook is present. The lower portion of central part is thinner than the upper part. Ray apophysis is clearly visible in scanning electron microscopy ( Fig. 2 C, 2D). Adoral–aboral axis of a single specimen showing bell-shaped body, adhesive disc, a portion of denticulate ring with radial pins and border membrane, blade apophysis, central conical part, central part clear ray apophysis in scanning electron microscopic study( Fig. 2 C, 2D). The end point of central part extends slightly towards y −1 axis ( Fig. 2 E, 2F).The section connecting anterior projection and central part is very prominent formed and curved towards the central area measuring 3.6–7.6 (5.8±2.8). The central part of the denticle is short and bent. The ray is short, stumpy and directed towards the center of an adhesive disc with a diameter of ray 1.0–2.5 (2.1±1.0), number of radial pins per denticle 5–6 (5.6±0.7), the number of denticles 22–24 (22.75±1.4), dimensions of denticle length 2–3 (2.5±0.7), blade 2.4–4.3 (3.3±1.3), span 4.1–6.1 (4.9±1.4). No significant variation in shape and structure of the denticles of the specimen has been found in the present studies with the findings of previous authors. The central part is triangular.

Taxonomic summary:

Host: Carassius auratus auratus (L.)

Locality : Haringhata, Nadia, West Bengal, India (22°53’N – 24°11’N / 88°09’E – 88°48’E)

Location: Gills

Reference material. Lecotype: In slide no. GF-TPB-5/2014, deposited at the collection of the Parasitology Laboratory, Department of Zoology, University of Kalyani P.O. Kalyani, Dist. Nadia, West Bengal, India.

Remarks. On analyzing the morphmetric and quantitative data recorded on the basis of the dry silver nitrate impregnation technique and scanning electron microscopic observations of the present specimen, it was readily identified as Tripartiella bulbosa ( Davis 1947; Lom 1959). The species was first described by Davis (1947) from West Virginia, USA . The ciliophoran obtained in the present investigation showed huge similarities to those recorded by Davis (1947) and Das & Haldar (1987) in West Bengal, India . Tripartiella bulbosa recovered from the gills and skin of Carassius auratus auratus (L.) for the first time from a biodiversity hot spot country like India is obviously far from complete. The present investigation extends this area to include the Carassius auratus auratus (L.) is added to the list of hosts.

Species T. bulbosa T. copicosa T. obtusa Host Carassius auratus auratus Carassius auratus auratus Carassius auratus auratus Locality Haringhata ,West HaringhataWest Haringhata, West

Bengal, India Bengal, India Bengal, India Site of Infection Gills Gills Gills Reference Present study Present study Present study Diameter of Body 18.0-23.4 (20.8±2.1) 18.4-25.2 (21.8±4.8 12.0-24.5 (18.25±8.8) Adhesive disc 13.2-18.7(16.7±3.8) 12.5-20.1 (16.3±5.3) 10.5-19.2 (14.85±6.1) Dimension of body

Denticular ring 6.4-9.3 (7.8±2.0) 6.6-9.5 (8.05±2.0) 7.1-9.7 (8.4±1.8)

Central area 3.6-7.6 (5.8±2.8) 3.1-6.1 4.6±2.1) 3.1-6.5 (4.8±2.4) Width of broader membrane 1.7-2.7 (2.2±0.7) 1.5-2.5 (2.1±07) 1.0-2.0 (1.5±0.7) Number of denticles 22-24 (22.75±1.4) 20-24 (22±2.8) 17-20 (18.5±2.1) Number of radial pins/Denticle 5-6 (5.6±0.7) 3-4 (3.5±0.7) 3-5 (4.0±1.4) Dimension of denticle

Span 4.1-6.1 (4.9±1.4) 4.0-5.5 (4.7±1.0) 5.5-8.0 (6.75±1.7)

Length 2-3 (2.5±0.7) 0.5-2.5 (1.5±1.4) 2.0-3.1(2.55±0.7) Dimension of denticle components

Length of ray 1.0-2.5 (2.1±1.0) 0.9-3.1 (2.0±1.5) 1.0-1.9 (1.45±0.6)

Length of blade 2.4-4.3 (3.3±1.3) 3.0-4.5 (3.75±1.0) 3.3-4.0 (3.65±0.4)

Width of central part 0.5-1.5 (1.0±0.7) 0.6-1.5(1.05±0.6) 0.5-1.5(1.0±0.7)

Figures

FIGURE 2. Microphotographs of Tripartiella bulbosa isolated from Carassius auratus auratus (L.) showing variation in appearance. (A & B) Showing the silver nitrate impregnated adhesive discs. Scale bars 10 µm (C & D) Showing the scanning electron micrographs of several Tripartiella bulbosa firmly attached to gill filaments. Scale Bar – 1 µm. (E & F) Diagrammatic drawings of the denticles of the species obtained in present study.