Saccharomyces cerevisiae

Umbraško, Inta, Batjuka, Anna, PetjukeviËs, Aleksandrs & - kute, NataÔja, 2022, EVALUATION OF THE EFFECTIVENESS OF USING A UNIVERSAL METHOD FOR ISOLATING GENOMIC dsDNA BY SALTING OUT TECHNIQUE ACCORDING TO THE S. M. ALJANABI AND I. MARTINEZ PROTOCOL FOR YEAST SACCHAROMYCES CEREVISIAE, Acta Biologica Universitatis Daugavpiliensis 22 (2), pp. 195-202 : 196

Cultivation of Saccharomyces cerevisiae

1 g of yeast ( Saccharomyces cerevisiae ) was inoculated into 9 ml of BRAIN HEART INFUSION BROTH (BioMaxima, Poland). Test tubes were placed in a thermostat for 24 hours at a temperature of +25°C. Then 1 ml of the inoculum was sown on a dense agar medium Plate Count Lab­ AGAR (BioMaxima, Poland) by surface method and cultivated at 25°C within 48 hours (Refrigerated Incubator FTC 90E). After 48 hours of cultivation, the culture gave abundant growth, the growth of typical of Saccharomyces cerevisiae : white, rounded, the surface is smooth, convex, shiny, the edges are even, homogeneous structure, mucous consistency, size with a diameter of 1–3 mm. Drying the drop at room temperature (+20), the preparation was fixed in a flame. Staining was carried out by microscopy of isolated colonies, the most commonly used stains for visible light microscopy are methylene blue, safranin, Lugol’s solution and tetrazolium chloride (Gram stain), immersion oil and examined with a 100x objective on a light microscope (Carl Zeiss stereomicroscope Axioscop 40).