Host

Reier, Susanne, Sattmann, Helmut, Schwaha, Thomas, Harl, Josef, Konecny, Robert & Haring, Elisabeth, 2019, An integrative taxonomic approach to reveal the status of the genus Pomphorhynchus Monticelli, 1905 (Acanthocephala: Pomphorhynchidae) in Austria, International Journal for Parasitology: Parasites and Wildlife 8, pp. 145-155 : 146

2.1. Host and parasite sampling

A total of 162 fish specimens from different rivers and lakes of

Styria, Vienna, Carinthia, Upper Austria and Lower Austria were examined ( Table 1). Fresh material was either preserved in 80% ethanol, frozen or dissected immediately after capture. Most of the fish intestines were derived from fish investigated during the Austrian Barcode of Life (ABOL) pilot study “DNA barcoding of Austrian vertebrates”. The parasites were obtained by dissection of fish intestines. Parasites were preserved in 80% ethanol and temporarily stored at 4 ̊C for further investigations. Specimens could be macroscopically assigned to the genus Pomphorhynchus based on the long neck and bulb. A total of 39 individuals of Pomphorhynchus ssp. were analyzed morphologically as well as genetically. In addition, nine specimens were analyzed only morphologically and 16 only genetically ( Table 1).

Generated DNA barcodes were uploaded to the Barcode of Life Database (BOLD) (Accession numbers: ACANT001-17, as well as ACANT002-18 – ACANT049-18).