Plutella xylostella (Linnaeus, 1767)

Zhang, Hong, Cheng, Chen, Li, Fengliang, Gu, Shuyu, Zhou, Zhou & Cheng, Luogen, 2013, Cloning and characterization of ubiquitin ribosome fusion gene RpS 27 a, a deltamethrin-resistance-associated gene from diamondback moth (Plutella xylostella L.), Turkish Journal of Zoology 37 (4), pp. 506-513 : 507

2.1. Plutella xylostella

P. xylostella was obtained from Huaxi cabbage fields (Guiyang, China). Toxicity evaluation showed that it was a deltamethrin-susceptible strain (LD 50 = 0.55 µg/larva). It was reared in our laboratory as the standard susceptible strain in this study.

The deltamethrin-resistant strain of P. xylostella was established and maintained by our laboratory selection (Liu et al., 1995), with continuous exposure to deltamethrin (Roussel-Uclaf, France) of the susceptible strain generation by generation as previously described (FAO, 1979). After more than 300 generations of laboratory selection, the resistance level was increased over 5000 times compared with the susceptible strain. Larvae of both strains were kept at 26 ± 1 °C with a constant photoperiod of 16 h light and 8 h dark and 75% relative humidity.