Paraphoma moravica Mlčoch, 2024

Mlčoch, Patrik & Matušinsky, Pavel, 2024, Phylogenetic and morphological revision sexual stages of the genus Paraphoma (Phaeosphaeriaceae) and next related species from clade of Ophiobolus-like (Phaeosphaeriaceae), Phytotaxa 663 (4), pp. 184-204 : 191

Paraphoma moravica Mlčoch , sp. nov. — Fig. 4

Holotype. BRNM 829157 .

MycoBank no: 854607

Etymology. The species name ¨moravica¨ is according to the locality of the first collection of type material, which is from the Moravia region.

Sexual morph. Ascomata pseudothecial, subglobose to globose, in numerous groups, subepidermal, 180–230 μm in diam.,100 μm, later semi-immersed to erumpent. Ostioles conical, to 35–50 μm height and 25–45 μm in diam. Substrate in surroundings of ascoma first uncoloured, later with characteristic purpure to light red coloured. Ascomal wall is composed of several layers of pseudoparenchymatous cells, composed of brown to dark brown polygonate cells of textura angularis, 5–6 × 4.5–5 μm, N = 20. Hamathecium is composed of numerous, indistinct, hyaline, septate pseudoparaphyses, anastomosed above the asci, later absent. Asci bitunicate, 8-spored, cylindrical to cylindric-clavate, short pedicellate, (71.5–)80–90(–100) × 7–7.5(–8) μm, N = 10. Ascospores narrowly fusiform, biseriate in the ascus, hyaline, 3-septate, the second cell from apex enlarged towards the base, slightly longer than wider, without gelatinous sheath, appendages and without ornamentation, with several globose lipid drops, (20.5–)21–27 × 4–5 μm, Q = 4.5–6.1, Q AV = 5.2, N = 20. Asexual morph. Sporulation in vitro after 2 weeks. Conidiomata in culture pycnidial, globose, up to 300 μm in diam., 150 μm high, with conical ostiole, 80 × 50–60 μm. Conidiomatal wall composed of several layers of brown, pseudoparenchymatous cells, 4.5–5 × 3–4.5 μm, N = 10. Conidiogenous cells were not observed. Conidia in culture was ellipsoid to fusoid, with rounded ends, hyaline, aseptate, smooth-walled, (5–)5.5–6.5(–7) × (1.5–)2–2.5(– 3) μm, Q = 2.3–3.1, Q AV = 2.7, N = 30.

Culture characters: Colony grown on PDA slow, 13–15 mm in diam. after 2 weeks at 25 ° C, circular to irregular, light grey at the edge, grey to dark grey at the centre, concentric coloured, later grey and not concentric coloured, entire to undulate at the edge, elevation flat to raise, no pigment product.

Habitat. Saprobic on dead stems of Dipsacus fullonum L. ( Caprifoliaceae ) in pond coastal vegetation.

Distribution. The Czech Republic.

Material examined: THE CZECH REPUBLIC. 1. col. P. Mlčoch, Moravian Gate, Jistebník, Sítinový rybník pond, 250 m. a. s. l., 11. 7. 2021, on the dead stems of Dipsacus fullonum, GPS : 49.7376503N, 18.1335128E. ( BRNM 829157, GenBank no. OQ359103.1, strain MLC 06, holotype).

Notes. ITS gene is 98.69%, similar to the taxon Paraphoma pye . However, isolate is phylogenetically related to Paraphoma vinacea and P. pye . These species, described by their asexual morph, grow on the other substrates ( P. pye and P. vinacea were isolated from Tanacetum cinerariifolium ( Asteraceae )—Moslemi A. et al. 2016; Moslemi A. et al. 2017) and has other microscopic characters in vitro ( P. pye on PDA has conidia 3.5–6.5 × 1.5–3 μm, oblong (Moslemi A. et al. 2017) and P. vinacea produces vinaceous red color on MEA, CHA and OA agar media and has ampulliform conidia 2–4.5 × 4–7 μm (Moslemi A. et al. 2017)). Sexual morph is morphologically related to Paraphoma rubicunda comb. nov. complex (see tab. 2). However, P. rubicunda has been found on the other hosts and is phylogenetically unrelated to the new species (fig. 1). Interestingly, Leptosphaeria bractearum (Sacc.) Sacc. , was also found on the dead stems of Dipsacus . Morphologically, L. bractearum can be distinguished from the new species in having wider asci (80–85 × 10–12 μm) with longer pedicel and smaller, 3-septate, yellow ascospores (18–20 × 5 μm) without the enlarged cell (Saccardo P. A., Traverso G. B., Trotter A. 1883).

Figures

FIGURE 4. Paraphoma moravica (BRNM 829157). A–B: ascoma; C: cultivate on PDA; D: ascomal wall; E: conidia in PDA culture; F–G: asci; H–K: ascospores. Scales:A–B: 500 μm; C: 30 mm; D–K: 10 μm.