Colletotrichum acutatum
publication ID |
https://doi.org/ 10.11646/phytotaxa.394.4.6 |
persistent identifier |
https://treatment.plazi.org/id/03AE6A28-AD3B-574A-FF31-FBC3FB52F9D9 |
treatment provided by |
Felipe |
scientific name |
Colletotrichum acutatum |
status |
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C. acutatum View in CoL species complex
For the 6-locus (ITS, GAPDH, CHS-1, HIS3, ACT, TUB2) phylogenetic analysis of the C. acutatum species complex, 38 reference sequences were downloaded from GenBank ( Table 1) and one isolate obtained from V. dunalianum var. urophyllum in this study. The multi-gene dataset (gene boundaries of ITS: 1−558, GAPDH: 559−811, CHS- 1: 812−1084, HIS3: 1085−1471, ACT: 1472−1697, TUB2: 1698−2187) comprised 2187 characters including the alignment gaps, of which 250 were parsimony-informative, 271 parsimony-uninformative and 1666 constant. The MP analysis of sequences resulted in one most parsimonious tree ( Fig. 1 View FIGURE 1 ) with a length (TL) of 886 steps, consistency index (CI) of 0.702, retention index (RI) of 0.844 and homoplasy index (HI) of 0.298. For the Bayesian analysis, MrModeltest 2.3 with the Akaike information criterion (AIC) was used to choose a substitution model for the two datasets. The model GRT+ I + G were chosen for the combined sequences. The analyses of the final MCMC chains were run from random trees for 1,000,000 generations and sampled every 100 generations. The tree generated from Bayesian analysis confirmed the tree topology obtained with parsimony. The phylogram constructed using combined dataset showed that V. dunalianum var. urophyllum isolate clustered into C. rhombiforme distinct clades with high bootstrap support (bootstrap support BS> 70 and Bayesian posterior probabilities PP> 0.95).
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