Osteocephalus leprieurii, Dumeril & Bibron, 1841

Ortiz, Diego A., Hoskin, Conrad J., Werneck, Fernanda P., Réjaud, AleXandre, Manzi, Sophie, Ron, Santiago R. & Fouquet, Antoine, 2023, Historical biogeography highlights the role of Miocene landscape changes on the diversification of a clade of Amazonian tree frogs, Organisms Diversity & Evolution (New York, N. Y.) 23 (2), pp. 395-414 : 408

Osteocephalus leprieurii group

The majority rule consensus delimited one OTU for the entire Osteocephalus leprieurii group ( Fig. 1a ). Two species have been formally described in this group: Osteocephalus leprieurii Duméril & Bibron, 1841 ( Fig. 5a–b in the Appendix) from the Guiana Shield; and O. yasuni Ron & Pramuk, 1999 ( Fig. 5c–d in the Appendix) from western Amazonia . Given these two species are phenotypically distinct (see ‘Diagnosis’ section in Ron and Pramuk (1999)), the low divergence in 16S led to not detecting them as different in the majority rule consensus. Therefore, we included a terminal for each in the mitogenomic matrix from nearby sites to their type localities. Although with low support, three sequences clustering within the O. yasuni clade from Boa Vista, south bank of upper Negro River (MTR41294), and from the south bank of lower Japura River (MTR33656, 33683), Amazonas, Brazil, correspond to its first molecular records in Central Amazonia (Electronic Supplements 3 and 4). Two additional lineages phenotypically similar to O. leprieurii have been reported and labelled as ‘ O. leprieurii [Ca1–2] UCS’ by Jungfer et al. (2013), but we did not include additional terminals in the mitogenomic matrix for them because of their current lack of morphological and bioacoustics information. We considered them here part of O. leprieurii ; however, further systematic study and more variable markers are needed to delimit species and population structure within this widespread taxon.

Figures

Fig. 1 Panel a, time-calibrated phylogeny of Osteocephalus, Tepuihyla and Dryaderces in Amazonia, resulting from a mitogenomic analysis in BEAST and displaying the mean and 95% CI (blue bars) of divergences. Nodes are numbered in red (age details are in Electronic Supplement 1), and support values are depicted for Bayesian (following node symbol colours) and maximum likelihood analyses (following numbers below the nodes). Topological discrepancies between analyses for four low-supported nodes (21, 25, 34 and 43)

Fig. 5 Dorsolateral views of Osteocephalus. a O. leprieurii sensu stricto, Kaw mountain, French Guiana. b O. leprieurii, AF1824, Mana, French Guiana. c O. yasuni sensu stricto, QCAZ69010, Estación Cientí- fica Yasuní, Orellana, Ecuador. d O. yasuni, QCAZ55994, Lorocachi, Pastaza, Ecuador. e O. deridens, QCAZ56031, Lorocachi, Pastaza, Ecuador. f O. fuscifacies, QCAZ59887, Parque Nacional Llanganates, Pastaza, Ecuador. g O. aff. leoniae 2, AF4513 (sequenced), San Martín, Peru. h O. plani- ceps, QCAZ55881, Lorocachi, Pastaza, Ecuador. Photographs: a–b, g by Antoine Fouquet; c–f, h by Santiago Ron (BIOWEB, Licence: CC BY-NC-ND 4.0)