Gint dabakalo, Kovarik & Mazuch, 2015

Just, Pavel, Šťáhlavský, František, Kovařík, František & Štundlová, Jana, 2022, Tracking the trends of karyotype differentiation in the phylogenetic context of Gint, a scorpion genus endemic to the Horn of Africa (Scorpiones: Buthidae), Zoological Journal of the Linnean Society 196 (2), pp. 885-901 : 890-891

GINT DABAKALO

In this species, the differences in the diploid number, multivalent associations and the appearance of 18S rDNA-bearing chromosomes are detected at inter- and intrapopulation levels, resulting in three distinguished cytotypes. Specimen S 1305 from the locality Burao is assigned to cytotype I, and specimens S1527 and S1199 from N of Burao, Togdheer represent cytotypes II and III, respectively .

Cytotype I (1 Ƌ; S1305) has 2 n = 23 chromosomes, which decrease in length from 7.90% to 2.55% of DSL (Supporting Information, Fig. S1D ; Table S2). The post-pachytene cells show nine bivalents and one pentavalent that is composed of chromosomes 1, 4, 5, 10 and 23 ( Fig. 2G ; Supporting Information, Fig. S1D ). The pair of 18S rDNA sites is located in the subterminal region of the largest bivalent ( Fig. 2H ).

Cytotype II (1 Ƌ; S1527) exhibits 2 n = 24 chromosomes, which decrease in length from 7.65% to 2.95% of DSL (Supporting Information, Fig. S1E ; Table S2). The post-pachytene nuclei show a configuration of eight bivalents, one trivalent (chromosomes 2, 12 and 17) and one pentavalent (chromosomes 1, 3, 4, 7 and 20) ( Fig. 2I ; Supporting Information, Fig. S1E ). The pair of 18S rDNA sites is situated in the subterminal region of chromosomes 2 and 12 of the trivalent ( Fig. 2J ; Supporting Information, Fig. S1E ).

Cytotype III (1 Ƌ; S1199) has 2 n = 27 chromosomes, which decrease in length from 8.30% to 1.80% of DSL (Supporting Information, Fig. S1F ; Table S2). In the post-pachytene spermatocytes, we observe eight bivalents, two trivalents and one pentavalent ( Fig. 2K ). The larger trivalent is composed of chromosomes 2, 9 and 10, the smaller trivalent is composed of chromosomes 4, 22 and 25, and chromosomes 1, 3, 5, 8 and 17 form a pentavalent (Supporting Information, Fig. S1F ). The 18S rDNA sites are located in the subterminal region of the chromosomes 2 and 9 of the larger trivalent ( Fig. 2L ; Supporting Information, Fig. S1F ).

Figures

Figure 1. Map showing distribution of the sampled Gint species. Bottom right – G. amoudensis.

Figure 2. Post-pachytene cells of Gint species after Giemsa staining (A, C, E, G, I, K, M, O) and FISH with 18S rDNA (red signals) (B, D, F, H, J,L, N, P).A, B,G. banfasae cytotype I(2n=18 –7II + IV).C, D, G. banfasae cytotype II (2n= 18 – 6II +VI). E, F, G. banfasae cytotype III (2n = 19 – 5II + III + VI). G, H, G. dabakalo cytotype I (2n = 23 – 9II + V). I, J,G. dabakalo cytotype II (2n = 24 – 8II + III + V). K, L, G. dabakalo cytotype III (2n = 27 – 8II + 2III + V). M, N, G. gaitako (2n = 30 – 13II + IV). O, P, G. maidensis (2n = 34). Abbreviations: II, bivalent; III, trivalent; IV, quadrivalent; V, pentavalent; VI, hexavalent. Arrowheads indicate the position of 18S rDNA. Scale bar = 10 µm.