Trichinella

Murakami, Masaki, Tokiwa, Toshihiro, Sugiyama, Hiromu, Shiroyama, Mitsuko, Morishima, Yasuyuki, Watanabe, Sota, Sasamori, Takato, Kondo, Mami, Mano, Tsutomu & Tsuruga, Hifumi, 2023, Trichinella T 9 in wild bears in Japan: Prevalence, species / genotype identification, and public health implications, International Journal for Parasitology: Parasites and Wildlife 21, pp. 264-268 : 266

3.2. Genetic analysis of Trichinella

A partial sequence of the ITS2 region (362 bp) was determined for all positive samples. Multiple sequence alignment revealed that seven sequences were 100% identical. A BLAST search revealed 100% sequence identity (362/362 bp) with Trichinella T9 detected in the brown bear (accession no. AB255886). The top three other species (query coverage> 95%) were T. britovi (<97.2%), T. murrelli (<96.1%), and Trichinella T6 (<95.2%).

A partial cox1 sequence (345 bp) was determined from the same positive samples, and these sequences were 100% identical. BLAST analysis revealed 100% sequence identity to that of Trichinella T9 from Japanese black bears from Iwate Prefecture (accession no. LC546041) and brown bears from Hokkaido (accession no. LC361217) and 99.7% of the sequences from Japanese black bears in Iwate Prefecture (accession no. LC546040) and raccoons from Hokkaido (accession no. AB267879). Two haplotypes have been reported in Japanese black bears, tentatively named h1 and h2, for descriptive purposes. However, in this study, only h1 was detected from two bear species in Hokkaido and Iwate. The other species with query coverage of> 95% was T. murrelli with <95.4%.

The representative sequences determined in this study have been deposited in the DNA Data Bank of Japan under accession nos. LC764456 (ITS2) and LC764591 (cox1).