Trichinella larvae

Murakami, Masaki, Tokiwa, Toshihiro, Sugiyama, Hiromu, Shiroyama, Mitsuko, Morishima, Yasuyuki, Watanabe, Sota, Sasamori, Takato, Kondo, Mami, Mano, Tsutomu & Tsuruga, Hifumi, 2023, Trichinella T 9 in wild bears in Japan: Prevalence, species / genotype identification, and public health implications, International Journal for Parasitology: Parasites and Wildlife 21, pp. 264-268 : 265

2.2. Detection of Trichinella larvae

A total of 20 g of the tongue muscle from each bear sample was excised, minced in a food processor, and artificially digested using a PrioCHECK Trichinella Alternative Artificial Digestion Kit (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’ s protocol. Trichinella larvae isolated from each sample in the digestants were mixed thoroughly with physiological saline in a plastic tube up to 50 mL. Subsequently, 5 mL of the solution was transferred to a 9 cm diameter plastic Petri dish. The number of larvae in each dish was counted thrice under a stereoscopic microscope, and the mean number of larvae per gram of tissue (LPG) was calculated. Differences in prevalence between bear species and sexes were separately examined using the chi-squared test.