Anagyrus aberiae Guerrieri, 2018

Guerrieri, Emilio & Cascone, Pasquale, 2018, Anagyrus Howard (Hymenoptera: Encyrtidae) parasitoids of the invasive Delottococcus aberiae (De Lotto) (Hemiptera: Pseudococcidae) from South Africa, with description of two new species, Zootaxa 4531 (3), pp. 374-382 : 376-378

Anagyrus aberiae Guerrieri sp. nov.

( Fig. 1–7 )

Description. Female (Holotype). Length, 1.6 mm. Head ( Fig. 1 ) and thorax orange, tegula whitish with brown apex; antenna ( Fig. 2 ) with radicle brown, scape largely brown except for two whitish bands at base and close to apex; pedicel and F1 brown at base and whitish at apex; remaining parts of antenna uniformly whitish; legs yellow with apices of tarsi brown; forewing hyaline, venation ( Fig. 3 ) brown; dorsum of gaster brown, sides and ventral part orange.

Head ( Fig. 1 ) on frotovertex between anterior ocellus and top of scrobes appearing smooth with extremely weak sculpture resembling that on mesoscutum; HW about 2.6× FV; ocelli forming an angle of about 90°; lowest margin of antennal torulus below lowest eye margin; antenna ( Fig. 2 ) with scape about 2× as long as wide, F1 about 0.6× as long as pedicel, funicule segments of about the same width and decreasing in length toward apex, F6 subquadrate, about 1.1× as long as broad. Relative measurements: HW 52, FV 20, POL 10, OOL 4, OCL 4, EL 34, EW 20, MS 10, SL 28, SW 14.

Mesoscutum 0.5× as long as broad and a little shorter than scutellum (13:12); forewing about 2.7× as long as broad, PMV ( Fig. 3 ) slightly shorter than MV; MV slightly shorter than SV. Relative measurements: FWL 130, FWW 50, MV 6, PMV 4, SV 8.

Metasoma about 1.3× as long as head and thorax together; ovipositor slightly exserted. Paratype: hypopygium ( Fig. 4 ) heart-shaped, 1.2×as wide as long; ovipositor ( Fig. 5 ) about 1.3× as long as mid tibia and 4.5× as long as gonostylus. Relative measurements: OL 127, MT 100, GL 27.

Male. Similar to female except for antenna ( Fig. 6 ) and genitalia. Scape about 4× as long as broad; pedicel subquadrate, all funicular segments longer than broad, their length decreasing towards apex, F1 4× as long as broad and about 3× as long as pedicel, F6 about 2.5× as long as broad; clava about 6× as long as broad and slightly longer than F5 and F6 together. Scale-like glands ( Guerrieri et al. 2001) ( Fig. 7 ) on F6 (5) clava (2). Relative measurements: SL 51, SW 13.

Variation. None in the material examined apart from one female being 2.1 mm in length.

Hosts. The species was reared from Delottococcus aberiae (De Lotto) ( Hemiptera : Pseudococcidae ) on hybrid grapefruit (Citrus x paradisi) ( Rutaceae ).

Distribution. South Africa.

Type material. Holotype ♀, South Africa, Limpopo province, 234518 m E 7357213 m S (= 23°52'28.5"S 30°23'34.7"E), 7.01.2017, ex Delottococcus aberiae (De Lotto) on Citrus x paradisi, Star Ruby (V. Martínez-Blay & J. Puig) (card-mounted) . Paratypes 5 ♀ ♀ (4 card-mounted, 2 slide-mounted), 3 ♂ ♂ (2 card-mounted, 2 slide- mounted), same data as holotype ; 1 ♀ (card-mounted), 1 ♂ (card-mounted), South Africa, Limpopo province, 238949 m E 7358974 m S (= 23°51'33.9"S 30°26'12.4"), 0 8.03.2017, ex Delottococcus aberiae (De Lotto) on Citrus x paradisi, Star Ruby (V. Martínez-Blay & J. Puig). Holotype and 3 paratypes deposited at NHM; 4 paratypes deposited at DEZA; 3 paratypes deposited at IAM-UPV. Sequences deposited in GenBank with the accession numbers: (Ang 001) MH 401216 ; (Ang002) MH 401217 ; (Ang004) MH 401218 ; (Ang005) MH 401219 ; (Ang006) MH 401220 .

Comments. Females of A. aberiae are similar to those A. pseudococci as defined in Trjapitsin et al. (2007) for body colour, pattern on scape, F1 having the base brown and the apex white, proportion of antennal segments. Apart from molecular evidence (see below and Fig. 18 for GenBank sequences examined), the two species can be separated by the proportion of HW and FV (2.6 in aberiae , 3.2 in pseudococci ), forewing proportions (2.7x as long as wide in aberiae , 2.3x in pseudococci ) and venation (MV distinctly longer than PMV and together longer than SV in aberiae , MV as long as PMV and together distinctly shorter than SV in pseudococci )

Figures

FIGURE 18. Tree based on COI sequences of closely related species of Anagyrus representing the maximum clade credibility tree with mean tree heights. Only posterior probabilities above 0.7 are displayed on the nodes.

FIGURES 1–7. Anagyrus aberiae Guerrieri sp. nov. ♀. 1. Head. 2. Antenna. 3. Fore wing venation. 4. Hypopygium. 5. Ovipositor. ♂. 6. Antenna. 7. Particular of male glands on F6 and clava.