Brienomyrus sp.

Ozouf-Costaz, Catherine, Coutanceau, Jean-Pierre, Bonillo, Céline, Belkadi, Laurent, Fermon, Yves, Agnese, Jean-François, Guidi-Rontani, Chantal & Paugy, Didier, 2015, First insights into karyotype evolution within the family Mormyridae, Cybium 39 (3), pp. 227-236 : 232

Brienomyrus sp.

The karyotype is composed of 2n = 50 (6sm + 2 m + 42a), FN = 58. C-bands are found in the centromeric regions of all chromosomes and no ITS was detected. The long arm of the third submetacentric is entirely C-band positive ( Fig. 3 , arrows). The 18S rDNA genes are located in terminal position on the long arms of submetacentric chromosomes (certainly corresponding to the third sm pair in size). These arms are DAPI-negative in their distal part ( Fig. 5A, B ). The telomeric probe produced weak signals in some telomeres, but not in all chromosomes ( Fig. 5C ). The microsatellite (GAA) 10 is abundant and mainly located in telomeric positions with few intercalary bands in pericentromeric position ( Fig. 5D ).

Figures

Figure 3. - Karyotypes of G. petersii, GNPE, M. moorii, MAMO, I. opdenboschi, IVOP, Brienomyrus sp., BR SP, S. walkeri, STWA, and P. microphthalmus, PEMI, after conventional Giemsa staining and C-banding. Scale bar = 10 µm.

Figure 5. - Metaphase plates of Brienomyrus sp. (A, B, C, D), S. walkeri (E, F, G, H) and P. microphthalmus (I, J, K, L), showing FISH patterns of the 18S rDNA probe, indirectly DIG-labelled, detected with rhodamine; DAPI counterstaining on the same metaphase plate; patterns of the Atto 488 labelled (TTAGGG)7 probe; patterns of the Cy3 labelled (GAA)10 satellite probe. Scale bars = 10 µm.