Ivindomyrus opdenboschi

Ozouf-Costaz, Catherine, Coutanceau, Jean-Pierre, Bonillo, Céline, Belkadi, Laurent, Fermon, Yves, Agnese, Jean-François, Guidi-Rontani, Chantal & Paugy, Didier, 2015, First insights into karyotype evolution within the family Mormyridae, Cybium 39 (3), pp. 227-236 : 231

Ivindomyrus opdenboschi

The karyotype is composed of 2n = 50 (2sm + 10m + 38a), FN = 62. A clear C-banding for this species could not be obtained: C-bands are found in the centromeric regions of all chromosomes and one band is located intercalary, in the long arms of the large sm pair ( Fig. 3 ). The 18S rDNA genes are located in the DAPI-negative short arms of a medium sized bi-armed element, but due to over-denaturation, it is difficult to assign it to a precise chromosome pair ( Fig. 4I, J ). The telomeric probe produced strong telomeric signals in all chromosomes but not all telomeres. In some acrocentric pairs, they can be located in long arms, pericentromeric position ( Fig. 4K ). The microsatellite (GAA) 10 is abundant and mainly located in telomeric positions with few intercalary bands in pericentromeric position ( Fig. 4L ).

Figures

Figure 3. - Karyotypes of G. petersii, GNPE, M. moorii, MAMO, I. opdenboschi, IVOP, Brienomyrus sp., BR SP, S. walkeri, STWA, and P. microphthalmus, PEMI, after conventional Giemsa staining and C-banding. Scale bar = 10 µm.

Figure 4. - Metaphase plates of G. petersii (A, B, C, D), M. moorii (E, F, G, H) and I. opdenboschi (I, J, K, L), showing FISH patterns of the 18S rDNA probe, indirectly DIG-labelled, detected with rhodamine; DAPI counterstaining on the same metaphase plate; patterns of the Atto 488 labeled (TTAGGG)7 probe; patterns of the Cy3 labeled (GAA)10 satellite probe. Scale bars = 10 µm.